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- W1992671702 abstract "A modified technique for the assay of progesterone in blood is presented. The procedure consists of three main steps: 1) ether extraction, 2) rapid and complete separation of progesterone from more polar steroids by the use of celite column chromatography, and 3) radioassay of progesterone by competitive protein binding. This assay method has a sensitivity of 0.1 ng and excellent specificity with low values for blank samples. The precision of this assay technique is comparable with other reported methods. This method offers two main advantages in comparison with previously described procedures. First, the celite column chromatography provides a rapid technique of separation of progesterone from other cross reacting steroids with a recovery of greater than 75 per cent. Secondly, the more polar steroids can be eluted subsequently and assayed separately. Thus, the assay of other steroids can be easily performed using the same serum extract." @default.
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- W1992671702 date "1971-01-01" @default.
- W1992671702 modified "2023-09-27" @default.
- W1992671702 title "A modified technique for the assay of progesterone in blood using celite column chromatography" @default.
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- W1992671702 doi "https://doi.org/10.1016/s0039-128x(71)80144-7" @default.
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