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- W1992709054 abstract "A simple method for preparing suspensions of free-floating neurons and neuroblasts from rat brain was described. Brains from newborn rats were torn in pieces with forceps and incubated with 0.1% trypsin. During the incubation dispersion of cells was helped by sucking the suspension in a pipette. Undissociated particles and tissue debris were removed by low-speed centrifugation. The yield was 70 to 80% on a protein, RNA or DNA, basis. The cells showed a protein/RNA ratio of 9.2 and a protein/DNA ratio of 19.1. The cells appeared intact in electron microscopy and 85% of them did not take up trypan blue. In a culture medium the cells maintained intact structure and protein synthetic activity for days." @default.
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- W1992709054 date "1972-12-01" @default.
- W1992709054 modified "2023-10-16" @default.
- W1992709054 title "Preparation of viable and morphologically intact cells from newborn rat brain" @default.
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- W1992709054 doi "https://doi.org/10.1016/0014-4827(72)90443-0" @default.
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