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- W1992752658 abstract "The particle size of Percoll, a polyvinylpyrrolidone-coated colloidal silica, has been analyzed by various techniques. The mean diameter of the dry particle from electron microscopy and from the molecular weight and partial specific volume is 21–22 nm. Hydrodynamic measurements (viscometry and sedimentation) give values of 29–30 and 35 nm in 0.15 M NaCl and water, respectively, indicating a layer of hydration on the particles which is more pronounced at low ionic strength. The second virial coefficient for Percoll in 0.15 M NaCl, as estimated from light scattering, osmometry, and sedimentation equilibrium, was 1.3–2.9 × 10−6. Assuming excluded volume to be the origin of the second virial coefficient one can calculate an effective particle diameter of 35–46 nm which is larger than the measured hydrodynamic diameter. Electrostatic forces do apparently contribute to the exclusion. The second virial coefficient in water is 100-fold larger than in 0.15 M NaCl. In the course of this investigation a technique was designed to measure sedimentation equilibria of concentrated Percoll solutions in a preparative zonal rotor. The colloid was concentrated in the bottom fraction of one run to 0.58 g/ml which is close to tight packing of the hydrated spheres. The material was still a clear fluid and no precipitate or gel was noticed during a long observation period." @default.
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- W1992752658 date "1980-07-01" @default.
- W1992752658 modified "2023-09-27" @default.
- W1992752658 title "Physical chemical characterization of percoll. II. Size and interaction of colloidal particles" @default.
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- W1992752658 doi "https://doi.org/10.1016/0021-9797(80)90278-7" @default.
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