Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992761701> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W1992761701 endingPage "671" @default.
- W1992761701 startingPage "669" @default.
- W1992761701 abstract "Alpha fetoprotein (AFP) is present at high concentrations in fetal fluids, certain neoplasias, and regenerating liver. Its physiological function remains largely unknown. Using a primary monolayer culture system, we investigated the proliferative activity of human (h) cord blood (CB) and highly purified AFP. hAFP, purified from hCB by Cibacron blue and immunoaffinity chromatography was homogeneous on SDS-PAGE and silver stain. Porcine granulosa cells from ovarian small follicles were cultured (25,000/cm2) for 2 days in medium (Ham's F-12:DMEM, 1:1) + 5% fetal calf serum (FCS) to facilitate attachment, followed by 6 days in medium containing: FCS, hCB or h amniotic fluid (1-20%)+/- EGF (10 ng/ml); or 0.25% plasma-derived serum (PDS) containing human low density lipoprotein (LDL, 25 ug/ml), +/- AFP (0.05-5 ug), and +/- EGF and IGF-I (10 ng/ml). In this system, single growth factors do not stimulate proliferation, a characteristic also exhibited by AFP. When combined with EGF, however, AFP dose-dependently increased proliferation to levels equal to that obtained with 10% FCS (2.3-fold increase vs PDS/LDL controls). When combined with EGF+IGF-I, AFP again dose-dependently increased proliferation to levels equal to that obtained with 10% FCS+EGF (6.7-fold increase vs controls). Purified human albumin used in place of AFP was not effective. TGF-a but not PDGF could replace the proliferative activity of EGF. These results suggest that AFP at physiological levels, although not itself mitogenic, can enhance the mitogenic activity of EGF and TGF-a and may function to modulate growth factor-mediated proliferation during development and neoplasia." @default.
- W1992761701 created "2016-06-24" @default.
- W1992761701 creator A5022165898 @default.
- W1992761701 creator A5051723908 @default.
- W1992761701 creator A5071691642 @default.
- W1992761701 date "1990-01-01" @default.
- W1992761701 modified "2023-09-23" @default.
- W1992761701 title "HUMAN ALPHA FETOPROTEIN ENHANCES EPIDERMAL GROWTH FACTOR PROLIFERATIVE ACTIVITY UPON PORCINE GRANDLOSA CELLS IN MONOLAYER CULTURE" @default.
- W1992761701 cites W1519656691 @default.
- W1992761701 cites W1570780771 @default.
- W1992761701 cites W1973303753 @default.
- W1992761701 cites W1990751555 @default.
- W1992761701 cites W2014347527 @default.
- W1992761701 cites W2026881410 @default.
- W1992761701 cites W2047386852 @default.
- W1992761701 cites W2079251901 @default.
- W1992761701 cites W2086987660 @default.
- W1992761701 cites W2588075432 @default.
- W1992761701 cites W322018090 @default.
- W1992761701 cites W2285027164 @default.
- W1992761701 cites W2306851665 @default.
- W1992761701 doi "https://doi.org/10.1210/endo-126-1-669" @default.
- W1992761701 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1688414" @default.
- W1992761701 hasPublicationYear "1990" @default.
- W1992761701 type Work @default.
- W1992761701 sameAs 1992761701 @default.
- W1992761701 citedByCount "29" @default.
- W1992761701 countsByYear W19927617012021 @default.
- W1992761701 crossrefType "journal-article" @default.
- W1992761701 hasAuthorship W1992761701A5022165898 @default.
- W1992761701 hasAuthorship W1992761701A5051723908 @default.
- W1992761701 hasAuthorship W1992761701A5071691642 @default.
- W1992761701 hasBestOaLocation W19927617011 @default.
- W1992761701 hasConcept C126322002 @default.
- W1992761701 hasConcept C134018914 @default.
- W1992761701 hasConcept C16685009 @default.
- W1992761701 hasConcept C170493617 @default.
- W1992761701 hasConcept C172680121 @default.
- W1992761701 hasConcept C185592680 @default.
- W1992761701 hasConcept C2775960820 @default.
- W1992761701 hasConcept C2776362946 @default.
- W1992761701 hasConcept C2778019345 @default.
- W1992761701 hasConcept C2779234561 @default.
- W1992761701 hasConcept C2780624289 @default.
- W1992761701 hasConcept C2780914630 @default.
- W1992761701 hasConcept C54355233 @default.
- W1992761701 hasConcept C71924100 @default.
- W1992761701 hasConcept C86803240 @default.
- W1992761701 hasConceptScore W1992761701C126322002 @default.
- W1992761701 hasConceptScore W1992761701C134018914 @default.
- W1992761701 hasConceptScore W1992761701C16685009 @default.
- W1992761701 hasConceptScore W1992761701C170493617 @default.
- W1992761701 hasConceptScore W1992761701C172680121 @default.
- W1992761701 hasConceptScore W1992761701C185592680 @default.
- W1992761701 hasConceptScore W1992761701C2775960820 @default.
- W1992761701 hasConceptScore W1992761701C2776362946 @default.
- W1992761701 hasConceptScore W1992761701C2778019345 @default.
- W1992761701 hasConceptScore W1992761701C2779234561 @default.
- W1992761701 hasConceptScore W1992761701C2780624289 @default.
- W1992761701 hasConceptScore W1992761701C2780914630 @default.
- W1992761701 hasConceptScore W1992761701C54355233 @default.
- W1992761701 hasConceptScore W1992761701C71924100 @default.
- W1992761701 hasConceptScore W1992761701C86803240 @default.
- W1992761701 hasIssue "1" @default.
- W1992761701 hasLocation W19927617011 @default.
- W1992761701 hasLocation W19927617012 @default.
- W1992761701 hasOpenAccess W1992761701 @default.
- W1992761701 hasPrimaryLocation W19927617011 @default.
- W1992761701 hasRelatedWork W1993795923 @default.
- W1992761701 hasRelatedWork W2002562299 @default.
- W1992761701 hasRelatedWork W2018368842 @default.
- W1992761701 hasRelatedWork W2021560856 @default.
- W1992761701 hasRelatedWork W2032639135 @default.
- W1992761701 hasRelatedWork W2091151050 @default.
- W1992761701 hasRelatedWork W2131811784 @default.
- W1992761701 hasRelatedWork W2141521254 @default.
- W1992761701 hasRelatedWork W2144562624 @default.
- W1992761701 hasRelatedWork W2156499933 @default.
- W1992761701 hasVolume "126" @default.
- W1992761701 isParatext "false" @default.
- W1992761701 isRetracted "false" @default.
- W1992761701 magId "1992761701" @default.
- W1992761701 workType "article" @default.