Matches in SemOpenAlex for { <https://semopenalex.org/work/W19927854> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W19927854 endingPage "303" @default.
- W19927854 startingPage "297" @default.
- W19927854 abstract "Cremophor EL (polyoxyethylene castor oil) and Tween 80, used as solvents for cyclosporin A and VP-16, respectively, were found to reverse the multidrug resistant (MDR) phenotype. In daunorubicin (DNR) resistant Ehrlich ascites tumor cells (EHR2/DNR+), both solvents at percentages of 0.01% (v/v) enhanced DNR accumulation to sensitive levels. Cremophor EL and Tween 80 did not influence DNR accumulation in drug-sensitive cells (EHR2). The concentration of cyclosporin A alone that enhanced DNR accumulation in EHR2/DNR+ cells to sensitive levels was 5 micrograms/mL whereas 0.2 micrograms/mL of cyclosporin A dissolved in 0.001% (v/v) Cremophor EL enhanced DNR accumulation to sensitive levels, thus indicating synergy between Cremophor EL and cyclosporin A. Cyclosporin A had a negligible effect on DNR accumulation in the drug-sensitive cells. In clonogenic assays, the LD10 of DNR was 1 microM in EHR2/DNR+ cells. Combining 1 microM DNR with non-toxic amounts of Cremophor EL (0.001% and 0.002%, v/v) potentiated the cytotoxicity of DNR and resulted in a cell kill of 77% and 86%, respectively, in the resistant cells. In non-toxic amounts, CrEL and Tween 80 acted synergistically with reduced concentrations of verapamil, resulting in DNR accumulation approaching close to the sensitive level. Azidopine photoaffinity labeling of P-glycoprotein in plasma membrane vesicles from EHR2/DNR+ cells was inhibited 100% and 80%, by 0.003% (v/v) Cremophor EL or Tween 80, respectively. These data permit the conclusion that non-toxic amounts of CrEL and Tween 80 modulated DNR resistance by raising intracellular DNR levels, due to their abilities to bind to the plasma membrane P-glycoprotein." @default.
- W19927854 created "2016-06-24" @default.
- W19927854 creator A5021340201 @default.
- W19927854 creator A5025868259 @default.
- W19927854 creator A5063711128 @default.
- W19927854 creator A5065012596 @default.
- W19927854 creator A5084893308 @default.
- W19927854 date "1990-01-01" @default.
- W19927854 modified "2023-10-01" @default.
- W19927854 title "The solvents cremophor EL and Tween 80 modulate daunorubicin resistance in the multidrug resistant Ehrlich ascites tumor." @default.
- W19927854 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1976341" @default.
- W19927854 hasPublicationYear "1990" @default.
- W19927854 type Work @default.
- W19927854 sameAs 19927854 @default.
- W19927854 citedByCount "36" @default.
- W19927854 countsByYear W199278542013 @default.
- W19927854 countsByYear W199278542017 @default.
- W19927854 countsByYear W199278542018 @default.
- W19927854 countsByYear W199278542019 @default.
- W19927854 countsByYear W199278542021 @default.
- W19927854 countsByYear W199278542022 @default.
- W19927854 countsByYear W199278542023 @default.
- W19927854 crossrefType "journal-article" @default.
- W19927854 hasAuthorship W19927854A5021340201 @default.
- W19927854 hasAuthorship W19927854A5025868259 @default.
- W19927854 hasAuthorship W19927854A5063711128 @default.
- W19927854 hasAuthorship W19927854A5065012596 @default.
- W19927854 hasAuthorship W19927854A5084893308 @default.
- W19927854 hasConcept C109316439 @default.
- W19927854 hasConcept C117262875 @default.
- W19927854 hasConcept C126322002 @default.
- W19927854 hasConcept C133936738 @default.
- W19927854 hasConcept C178790620 @default.
- W19927854 hasConcept C185592680 @default.
- W19927854 hasConcept C202751555 @default.
- W19927854 hasConcept C2776694085 @default.
- W19927854 hasConcept C2777022698 @default.
- W19927854 hasConcept C2781021840 @default.
- W19927854 hasConcept C2781303535 @default.
- W19927854 hasConcept C501593827 @default.
- W19927854 hasConcept C519063684 @default.
- W19927854 hasConcept C55493867 @default.
- W19927854 hasConcept C71924100 @default.
- W19927854 hasConcept C98274493 @default.
- W19927854 hasConceptScore W19927854C109316439 @default.
- W19927854 hasConceptScore W19927854C117262875 @default.
- W19927854 hasConceptScore W19927854C126322002 @default.
- W19927854 hasConceptScore W19927854C133936738 @default.
- W19927854 hasConceptScore W19927854C178790620 @default.
- W19927854 hasConceptScore W19927854C185592680 @default.
- W19927854 hasConceptScore W19927854C202751555 @default.
- W19927854 hasConceptScore W19927854C2776694085 @default.
- W19927854 hasConceptScore W19927854C2777022698 @default.
- W19927854 hasConceptScore W19927854C2781021840 @default.
- W19927854 hasConceptScore W19927854C2781303535 @default.
- W19927854 hasConceptScore W19927854C501593827 @default.
- W19927854 hasConceptScore W19927854C519063684 @default.
- W19927854 hasConceptScore W19927854C55493867 @default.
- W19927854 hasConceptScore W19927854C71924100 @default.
- W19927854 hasConceptScore W19927854C98274493 @default.
- W19927854 hasIssue "9" @default.
- W19927854 hasLocation W199278541 @default.
- W19927854 hasOpenAccess W19927854 @default.
- W19927854 hasPrimaryLocation W199278541 @default.
- W19927854 hasRelatedWork W1840819038 @default.
- W19927854 hasRelatedWork W1963779461 @default.
- W19927854 hasRelatedWork W2048313550 @default.
- W19927854 hasRelatedWork W2065723669 @default.
- W19927854 hasRelatedWork W2084401698 @default.
- W19927854 hasRelatedWork W2090794798 @default.
- W19927854 hasRelatedWork W2115367445 @default.
- W19927854 hasRelatedWork W2129571992 @default.
- W19927854 hasRelatedWork W2132081356 @default.
- W19927854 hasRelatedWork W2281118821 @default.
- W19927854 hasVolume "2" @default.
- W19927854 isParatext "false" @default.
- W19927854 isRetracted "false" @default.
- W19927854 magId "19927854" @default.
- W19927854 workType "article" @default.