FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992798511> ?p ?o ?g. }

• W1992798511 endingPage "56" @default.
• W1992798511 startingPage "45" @default.
• W1992798511 abstract "Immunization with a recombinant yeast-expressed Plasmodium falciparum merozoite surface protein 3 (MSP3) protected Aotus nancymai monkeys against a virulent challenge infection. Unfortunately, the production process for this yeast-expressed material was not optimal for human trials. In an effort to produce a recombinant MSP3 protein in a scaleable manner, we expressed and purified near-full-length MSP3 in Escherichia coli (EcMSP3). Purified EcMSP3 formed non-globular dimers as determined by analytical size-exclusion HPLC with in-line multi-angle light scatter and quasi-elastic light scatter detection and velocity sedimentation (R(h) 7.6+/-0.2nm and 6.9nm, respectively). Evaluation by high-resolution atomic force microscopy revealed non-linear asymmetric structures, with beaded domains and flexible loops that were recognized predominantly as dimers, although monomers and larger multimers were observed. The beaded substructure corresponds to predicted structural domains, which explains the velocity sedimentation results and improves the conceptual model of the protein. Vaccination with EcMSP3 in Freund's adjuvant-induced antibodies that recognized native MSP3 in parasitized erythrocytes by an immunofluorescence assay and gave delayed time to treatment in a group of Aotus monkeys in a virulent challenge infection with the FVO strain of P. falciparum. Three of the seven monkeys vaccinated with EcMSP3 had low peak parasitemias. EcMSP3, which likely mimics the native MSP3 structure located on the merozoite surface, is a viable candidate for inclusion in a multi-component malaria vaccine." @default.
• W1992798511 created "2016-06-24" @default.
• W1992798511 creator A5007663992 @default.
• W1992798511 creator A5013363685 @default.
• W1992798511 creator A5025309149 @default.
• W1992798511 creator A5028156362 @default.
• W1992798511 creator A5028623672 @default.
• W1992798511 creator A5039585773 @default.
• W1992798511 creator A5044942708 @default.
• W1992798511 creator A5052695583 @default.
• W1992798511 creator A5056139240 @default.
• W1992798511 creator A5056670181 @default.
• W1992798511 creator A5066170257 @default.
• W1992798511 creator A5066321691 @default.
• W1992798511 creator A5084472936 @default.
• W1992798511 date "2009-03-01" @default.
• W1992798511 modified "2023-09-27" @default.
• W1992798511 title "Characterization of a protective Escherichia coli-expressed Plasmodium falciparum merozoite surface protein 3 indicates a non-linear, multi-domain structure" @default.
• W1992798511 cites W1548178570 @default.
• W1992798511 cites W1554313068 @default.
• W1992798511 cites W1586351831 @default.
• W1992798511 cites W190349426 @default.
• W1992798511 cites W1975317507 @default.
• W1992798511 cites W1980581336 @default.
• W1992798511 cites W1986169276 @default.
• W1992798511 cites W1990440433 @default.
• W1992798511 cites W1998834739 @default.
• W1992798511 cites W2007107624 @default.
• W1992798511 cites W2019057969 @default.
• W1992798511 cites W2024645073 @default.
• W1992798511 cites W2026935151 @default.
• W1992798511 cites W2028506549 @default.
• W1992798511 cites W2040615064 @default.
• W1992798511 cites W2042659301 @default.
• W1992798511 cites W2045503393 @default.
• W1992798511 cites W2054291113 @default.
• W1992798511 cites W2060956856 @default.
• W1992798511 cites W2062166748 @default.
• W1992798511 cites W2067428749 @default.
• W1992798511 cites W2067559517 @default.
• W1992798511 cites W2094340197 @default.
• W1992798511 cites W2095477175 @default.
• W1992798511 cites W2102122585 @default.
• W1992798511 cites W2115150674 @default.
• W1992798511 cites W2135580377 @default.
• W1992798511 cites W2153187042 @default.
• W1992798511 cites W2163449711 @default.
• W1992798511 cites W2164128190 @default.
• W1992798511 cites W2164177269 @default.
• W1992798511 cites W2166262629 @default.
• W1992798511 cites W2168943316 @default.
• W1992798511 doi "https://doi.org/10.1016/j.molbiopara.2008.11.006" @default.
• W1992798511 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3633458" @default.
• W1992798511 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19073223" @default.
• W1992798511 hasPublicationYear "2009" @default.
• W1992798511 type Work @default.
• W1992798511 sameAs 1992798511 @default.
• W1992798511 citedByCount "19" @default.
• W1992798511 countsByYear W19927985112012 @default.
• W1992798511 countsByYear W19927985112013 @default.
• W1992798511 countsByYear W19927985112015 @default.
• W1992798511 countsByYear W19927985112016 @default.
• W1992798511 countsByYear W19927985112017 @default.
• W1992798511 countsByYear W19927985112019 @default.
• W1992798511 countsByYear W19927985112020 @default.
• W1992798511 crossrefType "journal-article" @default.
• W1992798511 hasAuthorship W1992798511A5007663992 @default.
• W1992798511 hasAuthorship W1992798511A5013363685 @default.
• W1992798511 hasAuthorship W1992798511A5025309149 @default.
• W1992798511 hasAuthorship W1992798511A5028156362 @default.
• W1992798511 hasAuthorship W1992798511A5028623672 @default.
• W1992798511 hasAuthorship W1992798511A5039585773 @default.
• W1992798511 hasAuthorship W1992798511A5044942708 @default.
• W1992798511 hasAuthorship W1992798511A5052695583 @default.
• W1992798511 hasAuthorship W1992798511A5056139240 @default.
• W1992798511 hasAuthorship W1992798511A5056670181 @default.
• W1992798511 hasAuthorship W1992798511A5066170257 @default.
• W1992798511 hasAuthorship W1992798511A5066321691 @default.
• W1992798511 hasAuthorship W1992798511A5084472936 @default.
• W1992798511 hasBestOaLocation W19927985112 @default.
• W1992798511 hasConcept C104317684 @default.
• W1992798511 hasConcept C12554922 @default.
• W1992798511 hasConcept C159047783 @default.
• W1992798511 hasConcept C203014093 @default.
• W1992798511 hasConcept C2778048844 @default.
• W1992798511 hasConcept C2778371730 @default.
• W1992798511 hasConcept C2781034427 @default.
• W1992798511 hasConcept C40767141 @default.
• W1992798511 hasConcept C54355233 @default.
• W1992798511 hasConcept C547475151 @default.
• W1992798511 hasConcept C86803240 @default.
• W1992798511 hasConceptScore W1992798511C104317684 @default.
• W1992798511 hasConceptScore W1992798511C12554922 @default.
• W1992798511 hasConceptScore W1992798511C159047783 @default.
• W1992798511 hasConceptScore W1992798511C203014093 @default.
• W1992798511 hasConceptScore W1992798511C2778048844 @default.
• W1992798511 hasConceptScore W1992798511C2778371730 @default.
• W1992798511 hasConceptScore W1992798511C2781034427 @default.

• next