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- W1992801579 abstract "Background: Quantum Dots (QDs) are fluorescent semiconductor nanocrystals that can replace the traditional fluorophores for various biomedical applications including cancer localisation, sentinel lymph node biopsy, detection of micrometastasis, image guided targeted drug delivery for chemotherapeutic agents and Photodynamic Therapy (PDT). QD technology forms one of the most promising frontiers in personalised medicine that would allow the diagnosis and treatment of disease at a truly molecular and cellular level. Their main limitation is toxicity, as most QDs are based on chalcogenide salts. We have synthesized and characterized a novel Polyhedral Oligomeric Silsesquioxane (POSS) coated QD using Mercaptosuccinic acid (MSA) and D-cysteine (D-cys) as stabilising agents and demonstrated its enhanced properties in an in vitro set up. POSS coated QDs were then conjugated to anti-Her-2 antibody and successfully used to localise Her-2 receptors on SKBR3 cells. Materials and Methods: QDs were synthesized using POSS/MSA/Dcys (POSS-QDs) or MSA/D-cys (MSA-QDs) by a one pot aqueous method. Characterization was performed using Transmission Electron Microscopy, Fourier Transform Infrared Spectroscopy and Photoluminescence studies. In vitro cytotoxicity was assessed using Hep G2 cells and compared to toxicity of ionised Cadmium and Tellurium salts. Photostability was assessed using prolonged excitation to high UV radiation for 2 hours. Confocal microscopy was used for QD localisation in vitro. POSS QDs were conjugated to mouse anti-Her2 antibody using cardodiimide chemistry and exposed to SKBR3 (Her-2 over expressing) and MCF-7 (Her-2 under expressing) breast cancer cells. Results: POSS-QDs had a size range of 3−4 nm and demonstrated enhanced colloidal stability and photostability (p-value <0.05) compared to MSA-QDs that became unstable on prolonged standing. FTIR confirmed the attachment of POSS to the CdTe core. Both QDs showed significantly reduced toxicity compared to ionised cadmium and tellurium (p-value <0.01). Confocal microscopy demonstrated enhanced intracellular uptake and photoluminescence of POSS-QDs at both 1 and 24 hours compared to MSA-QDs. POSS-QDs were significantly more photostable than MSA-QDs. Anti-Her-2 antibody conjugated POSS-QDs successfully localised Her-2 receptors on SKBR3 cells. Conclusion: A POSS coating confers photostability and colloidal stability while retaining the small size and unique photophysical properties of the QDs. The amphiphyllic nature of the POSS coating allows rapid intracellular uptake with enhanced photoluminescence allowing lower concentrations of QDs to be used for an overall reduced toxicity for various applications including Her-2 localisation and targeted cancer therapy." @default.
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- W1992801579 date "2012-03-01" @default.
- W1992801579 modified "2023-09-26" @default.
- W1992801579 title "110 A Novel Polyhedral Oligomeric Silsesquioxane Coated Quantum Dot for Her-2 Localisation and Cancer Therapy" @default.
- W1992801579 doi "https://doi.org/10.1016/s0959-8049(12)70178-8" @default.
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