Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992809929> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W1992809929 endingPage "14608" @default.
- W1992809929 startingPage "14598" @default.
- W1992809929 abstract "We focus on the assignment of function to and elucidation of structure−function relationships for a member of the mechanistically diverse enolase superfamily encoded by the Bradyrhizobium japonicum genome (bll6730; GI:27381841). As suggested by sequence alignments, the active site contains the same functional groups found in the active site of mandelate racemase (MR) that catalyzes a 1,1-proton transfer reaction: two acid/base catalysts, Lys 184 at the end of the second β-strand, and a His 322-Asp 292 dyad at the ends of the seventh and sixth β-strands, respectively, as well as ligands for an essential Mg2+, Asp 213, Glu 239, and Glu 265 at the ends of the third, fourth, and fifth β-strands, respectively. We screened a library of 46 acid sugars and discovered that only d-tartrate is dehydrated, yielding oxaloacetate as product. The kinetic constants (kcat = 7.3 s-1; kcat/KM = 8.5 × 104 M-1 s-1) are consistent with assignment of the d-tartrate dehydratase (TarD) function. The kinetic phenotypes of mutants as well as the structures of liganded complexes are consistent with a mechanism in which Lys 184 initiates the reaction by abstraction of the α-proton to generate a Mg2+-stabilized enediolate intermediate, and the vinylogous β-elimination of the 3-OH group is general acid-catalyzed by the His 322, accomplishing the anti-elimination of water. The replacement of the leaving group by solvent-derived hydrogen is stereorandom, suggesting that the enol tautomer of oxaloacetate is the product; this expectation was confirmed by its observation by 1H NMR spectroscopy. Thus, the TarD-catalyzed reaction is a “simple” extension of the two-step reaction catalyzed by MR: base-catalyzed proton abstraction to generate a Mg2+-stabilized enediolate intermediate followed by acid-catalyzed decomposition of that intermediate to yield the product." @default.
- W1992809929 created "2016-06-24" @default.
- W1992809929 creator A5006013523 @default.
- W1992809929 creator A5057267914 @default.
- W1992809929 creator A5061121659 @default.
- W1992809929 creator A5083499771 @default.
- W1992809929 creator A5085035303 @default.
- W1992809929 creator A5091252875 @default.
- W1992809929 date "2006-11-14" @default.
- W1992809929 modified "2023-09-27" @default.
- W1992809929 title "Evolution of Enzymatic Activities in the Enolase Superfamily: <scp>d</scp>-Tartrate Dehydratase from <i>Bradyrhizobium japonicum</i><sup>,</sup>" @default.
- W1992809929 cites W2034621209 @default.
- W1992809929 cites W2044736479 @default.
- W1992809929 cites W2053159677 @default.
- W1992809929 cites W2093990131 @default.
- W1992809929 cites W2112492958 @default.
- W1992809929 cites W3004422622 @default.
- W1992809929 doi "https://doi.org/10.1021/bi061688g" @default.
- W1992809929 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17144653" @default.
- W1992809929 hasPublicationYear "2006" @default.
- W1992809929 type Work @default.
- W1992809929 sameAs 1992809929 @default.
- W1992809929 citedByCount "31" @default.
- W1992809929 countsByYear W19928099292012 @default.
- W1992809929 countsByYear W19928099292013 @default.
- W1992809929 countsByYear W19928099292014 @default.
- W1992809929 countsByYear W19928099292015 @default.
- W1992809929 countsByYear W19928099292017 @default.
- W1992809929 countsByYear W19928099292018 @default.
- W1992809929 countsByYear W19928099292019 @default.
- W1992809929 countsByYear W19928099292021 @default.
- W1992809929 countsByYear W19928099292022 @default.
- W1992809929 countsByYear W19928099292023 @default.
- W1992809929 crossrefType "journal-article" @default.
- W1992809929 hasAuthorship W1992809929A5006013523 @default.
- W1992809929 hasAuthorship W1992809929A5057267914 @default.
- W1992809929 hasAuthorship W1992809929A5061121659 @default.
- W1992809929 hasAuthorship W1992809929A5083499771 @default.
- W1992809929 hasAuthorship W1992809929A5085035303 @default.
- W1992809929 hasAuthorship W1992809929A5091252875 @default.
- W1992809929 hasConcept C111233374 @default.
- W1992809929 hasConcept C118629725 @default.
- W1992809929 hasConcept C145148216 @default.
- W1992809929 hasConcept C178790620 @default.
- W1992809929 hasConcept C181199279 @default.
- W1992809929 hasConcept C185592680 @default.
- W1992809929 hasConcept C2781374117 @default.
- W1992809929 hasConcept C41183919 @default.
- W1992809929 hasConcept C55493867 @default.
- W1992809929 hasConcept C56856141 @default.
- W1992809929 hasConcept C71240020 @default.
- W1992809929 hasConceptScore W1992809929C111233374 @default.
- W1992809929 hasConceptScore W1992809929C118629725 @default.
- W1992809929 hasConceptScore W1992809929C145148216 @default.
- W1992809929 hasConceptScore W1992809929C178790620 @default.
- W1992809929 hasConceptScore W1992809929C181199279 @default.
- W1992809929 hasConceptScore W1992809929C185592680 @default.
- W1992809929 hasConceptScore W1992809929C2781374117 @default.
- W1992809929 hasConceptScore W1992809929C41183919 @default.
- W1992809929 hasConceptScore W1992809929C55493867 @default.
- W1992809929 hasConceptScore W1992809929C56856141 @default.
- W1992809929 hasConceptScore W1992809929C71240020 @default.
- W1992809929 hasIssue "49" @default.
- W1992809929 hasLocation W19928099291 @default.
- W1992809929 hasLocation W19928099292 @default.
- W1992809929 hasOpenAccess W1992809929 @default.
- W1992809929 hasPrimaryLocation W19928099291 @default.
- W1992809929 hasRelatedWork W1499619215 @default.
- W1992809929 hasRelatedWork W1976314208 @default.
- W1992809929 hasRelatedWork W2006946696 @default.
- W1992809929 hasRelatedWork W2078755351 @default.
- W1992809929 hasRelatedWork W2084240653 @default.
- W1992809929 hasRelatedWork W2155257397 @default.
- W1992809929 hasRelatedWork W2342448571 @default.
- W1992809929 hasRelatedWork W2789432453 @default.
- W1992809929 hasRelatedWork W2807437747 @default.
- W1992809929 hasRelatedWork W4205384157 @default.
- W1992809929 hasVolume "45" @default.
- W1992809929 isParatext "false" @default.
- W1992809929 isRetracted "false" @default.
- W1992809929 magId "1992809929" @default.
- W1992809929 workType "article" @default.