FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992811538> ?p ?o ?g. }

• W1992811538 endingPage "493" @default.
• W1992811538 startingPage "493" @default.
• W1992811538 abstract "A biosynthesis method was developed to produce a standard of a selenium-containing protein. It consisted of the expression of calmodulin in Escherichia coli culture in the presence of selenomethionine, which allowed the replacement of all methionine residues by selenomethionine. The resulting 17 kDa protein containing 8 selenomethionine residues was purified by two-step hydrophobic interaction chromatography. The selenomethionyl calmodulin was subsequently used to develop a method for the characterization of selenium-containing proteins (detected in the polyacrylamide gel by laser ablation-ICP-MS) by means of peptide mapping using capillary HPLC-ICP-MS. The monitoring of the 80Se isotope using an ICP mass spectrometer equipped with a collision cell allowed as little as 0.3 pg as Se (1.3 ng ml−1 in the analysed solution) to be detected in the gel. The band containing the protein of interest was excised, the protein was digested with trypsin and the Se-containing peptides were analyzed by capillary HPLC-ICP-MS. The sensitivity of the method was at least a factor of 5 higher than that of capillary LC-electrospray MS/MS in similar conditions. Some of the selenopeptides detected by capillary LC-ICP MS could nevertheless be identified by retention time matching using a set of peptides generated by trypsin digestion from the concentrated selenomethionyl calmodulin standard." @default.
• W1992811538 created "2016-06-24" @default.
• W1992811538 creator A5017511524 @default.
• W1992811538 creator A5021083749 @default.
• W1992811538 creator A5027213349 @default.
• W1992811538 creator A5054421409 @default.
• W1992811538 creator A5060790483 @default.
• W1992811538 creator A5067114512 @default.
• W1992811538 creator A5090198427 @default.
• W1992811538 date "2005-01-01" @default.
• W1992811538 modified "2023-10-11" @default.
• W1992811538 title "Biosynthesis, purification and analysis of selenomethionyl calmodulin by gel electrophoresis-laser ablation-ICP-MS and capillary HPLC-ICP-MS peptide mapping following in-gel tryptic digestion" @default.
• W1992811538 cites W1481302364 @default.
• W1992811538 cites W1966544705 @default.
• W1992811538 cites W1975920595 @default.
• W1992811538 cites W1978038282 @default.
• W1992811538 cites W1985773762 @default.
• W1992811538 cites W1988599936 @default.
• W1992811538 cites W1989112108 @default.
• W1992811538 cites W2007091221 @default.
• W1992811538 cites W2008028760 @default.
• W1992811538 cites W2024554691 @default.
• W1992811538 cites W2031544072 @default.
• W1992811538 cites W2032832593 @default.
• W1992811538 cites W2033497910 @default.
• W1992811538 cites W2034383704 @default.
• W1992811538 cites W2046276400 @default.
• W1992811538 cites W2058852380 @default.
• W1992811538 cites W2069187446 @default.
• W1992811538 cites W2070219878 @default.
• W1992811538 cites W2070228646 @default.
• W1992811538 cites W2083086215 @default.
• W1992811538 cites W2089258642 @default.
• W1992811538 cites W2095911028 @default.
• W1992811538 cites W2100837269 @default.
• W1992811538 cites W2101980423 @default.
• W1992811538 cites W2120623166 @default.
• W1992811538 cites W2135579153 @default.
• W1992811538 cites W2136725886 @default.
• W1992811538 doi "https://doi.org/10.1039/b500719d" @default.
• W1992811538 hasPublicationYear "2005" @default.
• W1992811538 type Work @default.
• W1992811538 sameAs 1992811538 @default.
• W1992811538 citedByCount "34" @default.
• W1992811538 countsByYear W19928115382012 @default.
• W1992811538 countsByYear W19928115382013 @default.
• W1992811538 countsByYear W19928115382014 @default.
• W1992811538 countsByYear W19928115382015 @default.
• W1992811538 countsByYear W19928115382016 @default.
• W1992811538 countsByYear W19928115382020 @default.
• W1992811538 countsByYear W19928115382021 @default.
• W1992811538 crossrefType "journal-article" @default.
• W1992811538 hasAuthorship W1992811538A5017511524 @default.
• W1992811538 hasAuthorship W1992811538A5021083749 @default.
• W1992811538 hasAuthorship W1992811538A5027213349 @default.
• W1992811538 hasAuthorship W1992811538A5054421409 @default.
• W1992811538 hasAuthorship W1992811538A5060790483 @default.
• W1992811538 hasAuthorship W1992811538A5067114512 @default.
• W1992811538 hasAuthorship W1992811538A5090198427 @default.
• W1992811538 hasConcept C162356407 @default.
• W1992811538 hasConcept C178790620 @default.
• W1992811538 hasConcept C179998833 @default.
• W1992811538 hasConcept C181199279 @default.
• W1992811538 hasConcept C185592680 @default.
• W1992811538 hasConcept C2779281246 @default.
• W1992811538 hasConcept C2780340462 @default.
• W1992811538 hasConcept C2900643 @default.
• W1992811538 hasConcept C29688787 @default.
• W1992811538 hasConcept C31827203 @default.
• W1992811538 hasConcept C43617362 @default.
• W1992811538 hasConcept C553756173 @default.
• W1992811538 hasConcept C55493867 @default.
• W1992811538 hasConceptScore W1992811538C162356407 @default.
• W1992811538 hasConceptScore W1992811538C178790620 @default.
• W1992811538 hasConceptScore W1992811538C179998833 @default.
• W1992811538 hasConceptScore W1992811538C181199279 @default.
• W1992811538 hasConceptScore W1992811538C185592680 @default.
• W1992811538 hasConceptScore W1992811538C2779281246 @default.
• W1992811538 hasConceptScore W1992811538C2780340462 @default.
• W1992811538 hasConceptScore W1992811538C2900643 @default.
• W1992811538 hasConceptScore W1992811538C29688787 @default.
• W1992811538 hasConceptScore W1992811538C31827203 @default.
• W1992811538 hasConceptScore W1992811538C43617362 @default.
• W1992811538 hasConceptScore W1992811538C553756173 @default.
• W1992811538 hasConceptScore W1992811538C55493867 @default.
• W1992811538 hasIssue "6" @default.
• W1992811538 hasLocation W19928115381 @default.
• W1992811538 hasLocation W19928115382 @default.
• W1992811538 hasLocation W19928115383 @default.
• W1992811538 hasOpenAccess W1992811538 @default.
• W1992811538 hasPrimaryLocation W19928115381 @default.
• W1992811538 hasRelatedWork W1970247801 @default.
• W1992811538 hasRelatedWork W2157021403 @default.
• W1992811538 hasRelatedWork W2186778521 @default.
• W1992811538 hasRelatedWork W2362495296 @default.
• W1992811538 hasRelatedWork W2369164738 @default.
• W1992811538 hasRelatedWork W2370903789 @default.
• W1992811538 hasRelatedWork W2376746905 @default.

• next