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- W199281666 abstract "Objective To establish a rapid, sensitive and specific SYBR Green real-time Copro-PCR method to detect Schistosoma japonicum infection and to evaluate the sensitivity of this method. Methods The artificial positive fecal samples were prepared by mixing counted schistosomal eggs with fecal samples of healthy buffalos. DNA templates for SYBR Green real-time PCR reaction were extracted from the artificial positive fecal samples using QIAamp DNA Stool Kit with modified protocols. The relationship between the logarithmic transformated egg counts of artificial positive fecal samples and Ct values was studied. For quality control, quantitative PCR reactions using the DNA templates extracted from eggs only and the normal saline (without fecal samples) were also performed throughout the procedure. Results This real-time PCR method is sensitive to detecting 1 schistosomal egg in 200 mg fecal samples. A linear regression was established between Ct values and eggs per gram (EPG) of feces. Conclusion The SYBR Green real-time PCR method is a highly sensitive copro-method to detect S. japonicum infection. A linear relationship is defined between the logarithmic transformated EPG and Ct values." @default.
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- W199281666 date "2009-01-01" @default.
- W199281666 modified "2023-09-29" @default.
- W199281666 title "Sensitivity of SYBR Green real-time copro-PCR on detection of Schistosoma japonicum infection." @default.
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