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- W1992833404 abstract "The gene encoding the Mycobacterium tuberculosis Rv2536 protein is present in the Mycobacterium tuberculosis complex (as assayed by PCR) and transcribed (as determined by RT-PCR) in M. tuberculosis H37Rv, M. tuberculosis H37Ra, M. bovis BCG, and M. africanum strains. Rabbits immunized with synthetic polymer peptides from this protein produced antibodies specifically recognizing a 25-kDa band in mycobacterial sonicate. U937 and A549 cells were used in binding assays involving 20-amino-acid-long synthetic peptides covering the whole Rv2536 protein sequence. Peptide 11207 (161DVFSAVRADDSPTGEMQVAQY180) presented high specific binding to both types of cells; the binding was saturable and presented nanomolar affinity constants. Cross-linking assays revealed that this peptide specifically binds to 50 kDa U937 cell membrane and 45 kDa A549 cell membrane proteins." @default.
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- W1992833404 date "2005-09-01" @default.
- W1992833404 modified "2023-10-18" @default.
- W1992833404 title "<i>Mycobacterium tuberculosis</i> Rv2536 protein implicated in specific binding to human cell lines" @default.
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- W1992833404 doi "https://doi.org/10.1110/ps.051526305" @default.
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