FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1992836740> ?p ?o ?g. }

• W1992836740 abstract "Abstract Background Aneuploidy is a feature of most cancer cells that is often accompanied by an elevated rate of chromosome mis-segregation termed chromosome instability (CIN). While CIN can act as a driver of cancer genome evolution and tumor progression, recent findings point to the existence of a threshold level beyond which CIN becomes a barrier to tumor growth and therefore can be exploited therapeutically. Drugs known to increase CIN beyond the therapeutic threshold are currently few in number, and the clinical promise of targeting the CIN phenotype warrants new screening efforts. However, none of the existing methods, including the in vitro micronuclei (MNi) assay, developed to quantify CIN, is entirely satisfactory. Methods We have developed a new assay for measuring CIN. This quantitative assay for chromosome mis-segregation is based on the use of a non-essential human artificial chromosome (HAC) carrying a constitutively expressed EGFP transgene. Thus, cells that inherit the HAC display green fluorescence, while cells lacking the HAC do not. This allows the measurement of HAC loss rate by routine flow cytometry. Results Using the HAC-based chromosome loss assay, we have analyzed several well-known anti-mitotic, spindle-targeting compounds, all of which have been reported to induce micronuclei formation and chromosome loss. For each drug, the rate of HAC loss was accurately measured by flow cytometry as a proportion of non-fluorescent cells in the cell population which was verified by FISH analysis. Based on our estimates, despite their similar cytotoxicity, the analyzed drugs affect the rates of HAC mis-segregation during mitotic divisions differently. The highest rate of HAC mis-segregation was observed for the microtubule-stabilizing drugs, taxol and peloruside A. Conclusion Thus, this new and simple assay allows for a quick and efficient screen of hundreds of drugs to identify those affecting chromosome mis-segregation. It also allows ranking of compounds with the same or similar mechanism of action based on their effect on the rate of chromosome loss. The identification of new compounds that increase chromosome mis-segregation rates should expedite the development of new therapeutic strategies to target the CIN phenotype in cancer cells." @default.
• W1992836740 created "2016-06-24" @default.
• W1992836740 creator A5017893931 @default.
• W1992836740 creator A5018512578 @default.
• W1992836740 creator A5028261479 @default.
• W1992836740 creator A5043961547 @default.
• W1992836740 creator A5045506614 @default.
• W1992836740 creator A5047873642 @default.
• W1992836740 creator A5053432642 @default.
• W1992836740 creator A5060435628 @default.
• W1992836740 creator A5075550817 @default.
• W1992836740 creator A5084764847 @default.
• W1992836740 date "2013-05-22" @default.
• W1992836740 modified "2023-10-10" @default.
• W1992836740 title "A new assay for measuring chromosome instability (CIN) and identification of drugs that elevate CIN in cancer cells" @default.
• W1992836740 cites W1924427056 @default.
• W1992836740 cites W1949152416 @default.
• W1992836740 cites W1984599124 @default.
• W1992836740 cites W1986395573 @default.
• W1992836740 cites W2003880075 @default.
• W1992836740 cites W2004852601 @default.
• W1992836740 cites W2006882273 @default.
• W1992836740 cites W2009502142 @default.
• W1992836740 cites W2017046792 @default.
• W1992836740 cites W2020099456 @default.
• W1992836740 cites W2023200988 @default.
• W1992836740 cites W2029039908 @default.
• W1992836740 cites W2029770190 @default.
• W1992836740 cites W2037404897 @default.
• W1992836740 cites W2042107535 @default.
• W1992836740 cites W2044993463 @default.
• W1992836740 cites W2045897698 @default.
• W1992836740 cites W2065826574 @default.
• W1992836740 cites W2068577449 @default.
• W1992836740 cites W2071393652 @default.
• W1992836740 cites W2090197496 @default.
• W1992836740 cites W2093855538 @default.
• W1992836740 cites W2095617425 @default.
• W1992836740 cites W2097867892 @default.
• W1992836740 cites W2111168474 @default.
• W1992836740 cites W2112784018 @default.
• W1992836740 cites W2122004639 @default.
• W1992836740 cites W2127279305 @default.
• W1992836740 cites W2141805323 @default.
• W1992836740 cites W2152394330 @default.
• W1992836740 cites W2153304690 @default.
• W1992836740 cites W2159708914 @default.
• W1992836740 cites W2171175279 @default.
• W1992836740 cites W2172046446 @default.
• W1992836740 cites W2344497449 @default.
• W1992836740 doi "https://doi.org/10.1186/1471-2407-13-252" @default.
• W1992836740 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3671967" @default.
• W1992836740 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23694679" @default.
• W1992836740 hasPublicationYear "2013" @default.
• W1992836740 type Work @default.
• W1992836740 sameAs 1992836740 @default.
• W1992836740 citedByCount "34" @default.
• W1992836740 countsByYear W19928367402014 @default.
• W1992836740 countsByYear W19928367402016 @default.
• W1992836740 countsByYear W19928367402017 @default.
• W1992836740 countsByYear W19928367402018 @default.
• W1992836740 countsByYear W19928367402019 @default.
• W1992836740 countsByYear W19928367402020 @default.
• W1992836740 countsByYear W19928367402021 @default.
• W1992836740 countsByYear W19928367402022 @default.
• W1992836740 crossrefType "journal-article" @default.
• W1992836740 hasAuthorship W1992836740A5017893931 @default.
• W1992836740 hasAuthorship W1992836740A5018512578 @default.
• W1992836740 hasAuthorship W1992836740A5028261479 @default.
• W1992836740 hasAuthorship W1992836740A5043961547 @default.
• W1992836740 hasAuthorship W1992836740A5045506614 @default.
• W1992836740 hasAuthorship W1992836740A5047873642 @default.
• W1992836740 hasAuthorship W1992836740A5053432642 @default.
• W1992836740 hasAuthorship W1992836740A5060435628 @default.
• W1992836740 hasAuthorship W1992836740A5075550817 @default.
• W1992836740 hasAuthorship W1992836740A5084764847 @default.
• W1992836740 hasBestOaLocation W19928367401 @default.
• W1992836740 hasConcept C104317684 @default.
• W1992836740 hasConcept C121608353 @default.
• W1992836740 hasConcept C126322002 @default.
• W1992836740 hasConcept C140752955 @default.
• W1992836740 hasConcept C143425029 @default.
• W1992836740 hasConcept C153911025 @default.
• W1992836740 hasConcept C178169997 @default.
• W1992836740 hasConcept C2777542201 @default.
• W1992836740 hasConcept C2779672484 @default.
• W1992836740 hasConcept C2908647359 @default.
• W1992836740 hasConcept C29730261 @default.
• W1992836740 hasConcept C30481170 @default.
• W1992836740 hasConcept C45080847 @default.
• W1992836740 hasConcept C502942594 @default.
• W1992836740 hasConcept C54355233 @default.
• W1992836740 hasConcept C552990157 @default.
• W1992836740 hasConcept C553184892 @default.
• W1992836740 hasConcept C71924100 @default.
• W1992836740 hasConcept C86803240 @default.
• W1992836740 hasConcept C93304396 @default.
• W1992836740 hasConcept C95444343 @default.
• W1992836740 hasConcept C99454951 @default.
• W1992836740 hasConceptScore W1992836740C104317684 @default.

• next