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- W1992964059 abstract "Thin sections of deep-frozen unfixed muscle were studied in a scanning electron microscope modified for transmission imaging and equipped with a “cryostage” for vacuum compatibility of hydrated tissue. With an energy-dispersive x-ray analysis system, intracellular atomic species in the scan beam path were identified by their fluorescent x-rays and spatially localized in correlation with the electron optical image of the microstructure. Marked differences are noted between the ultrastructure of deep-frozen hydrated muscle and that of fixed dehydrated muscle. In frozen muscle, myofibrils appear to be composed of previously undescribed longitudinal structures between 400-1000 Å wide (“macromyofilaments”). The usual myofilaments, mitochondria, and sarcoplasmic reticulum were not seen unless the tissue was “fixed” before examination. Fluorescent x-ray analysis of the spatial location of constituent elements clearly identified all elements heavier than Na. Intracellular Cl was relatively higher than expected." @default.
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- W1992964059 date "1973-12-01" @default.
- W1992964059 modified "2023-09-26" @default.
- W1992964059 title "Scanning Transmission Electron microscope Studies of Deep-Frozen Unfixed Muscle Correlated with Spatial Localization of Intracellular Elements by Fluorescent X-Ray Analysis" @default.
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- W1992964059 doi "https://doi.org/10.1073/pnas.70.12.3423" @default.
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