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- W1992977897 abstract "The growth physiology ofSaccharomyces cerevisiae strains H1022 and Whi2+ has been studied in aerobic batch and continuous (chemostat) cultures. Results from the measurement of biomass and medium components (off-line) together with oxygen, carbon dioxide and heat measurements (on-line) have been used in an attempt to explore the existence of ‘overflow’ or ‘bottleneck’ metabolism as opposed to catabolite repression (Crabtree effect) in these strains. Chemostat experiments indicated that specific oxygen uptake rate (qo2) was linearly related to the dilution rate (D) at values below the critical dilution rate (Dcrit), becoming constant aboveDcrit, which is in agreement with the bottleneck theory. However, batch culture experiments indicated negligible oxygen consumption during the initial glucose growth phase, the culture exhibiting purely anaerobic metabolism. The bottleneck theory would propose thatqO2, has a constant (maximum) value under these conditions. The results presented here suggest that while the bottleneck theory can be adequately used to describe chemostat growth ofS. cerevisiae, some other control mechanism must be operating under conditions of high glucose concentrations, such as those initially prevailing in the batch culture experiments." @default.
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- W1992977897 date "1989-12-01" @default.
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- W1992977897 title "Further evidence for the existence of a bottleneck in the metabolism ofSaccharomyces cerevisiae" @default.
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- W1992977897 doi "https://doi.org/10.1007/bf01950151" @default.
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