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- W1993048802 abstract "Properties of the matrix and stationary phase which affect the intrinsic loading capacity of silica-based packing materials for preparative anion-exchange chromatography of proteins were investigated. Polyethyleneimine-coated controlled porosity glass beads ranging from 100 to 2000 A in pore diameter were used to evaluate the effects of pore diameter and surface area. Protein binding was found to depend on accessible, rather than total, support surface area. Consequently, wide-pore, high surface area media provide maximum intrinsic loading capacity. Increasing the number of positively charged sites on the stationary phase by increased coating or by quaternization of amines increases hemoglobin-binding capacity." @default.
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- W1993048802 date "1986-01-01" @default.
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- W1993048802 title "Factors contributing to intrinsic loading capacity in silica-based packing materials for preparative anion-exchange protein chromatography" @default.
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- W1993048802 doi "https://doi.org/10.1016/s0021-9673(01)86967-3" @default.
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