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• W1993191507 abstract "Four series of neuroblastoma cell hybrids were isolated, using as parental cells a human and a mouse neuroblastoma, a human fibroblast, and a mouse L cell line. Hybrid clones were analyzed for karyotype, morphology, acetylcholinesterase, and choline acetyltransferase. Acetylcholinesterase activity, which was present in the neuroblastoma parents but absent in the fibroblasts, showed continued expression in all series of hybrids, and neuronal morphology was expressed in three of the four hybrid series. Choline acetyltransferase, which mediates acetylcholine synthesis in cholinergic neurons, was absent from all parents and hybrids except for one hybrid clone, in which expression of the enzyme was activated. These results are compared with the results, reported elsewhere, of assays of the same hybrids for additional neuronal phenotypes. These phenotypes are electrical excitability and acetylcholine sensitivity, presence of the neuron-specific 14-3-2 protein and steroid sulfatase, and glycosphingolipd composition. Among those phenotypes which continue to be expressed, the level of expression is closely correlated with acetylcholinesterase specific activity, and also with chromosome number. These results are discussed in terms of the genetic regulation of cell differentiation." @default.
• W1993191507 created "2016-06-24" @default.
• W1993191507 creator A5012816784 @default.
• W1993191507 creator A5089701671 @default.
• W1993191507 date "1974-08-01" @default.
• W1993191507 modified "2023-10-04" @default.
• W1993191507 title "Expression of neuronal phenotypes in neuroblastoma cell hybrids" @default.
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• W1993191507 doi "https://doi.org/10.1016/0012-1606(74)90237-1" @default.
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