Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993211567> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W1993211567 endingPage "2553" @default.
- W1993211567 startingPage "2541" @default.
- W1993211567 abstract "The protein kinase C (PKC) binding site used by PKC activators such as phorbol esters and diacylglycerols (DAGs) has been characterized by means of molecular modeling and site-directed mutagenesis studies. Based upon a NMR-determined solution structure of the second cysteinerich domain of PKC alpha, molecular modeling was used to study the structures of the complexes formed between the PKC receptor and a number of PKC ligands, phorbol esters, and DAGs. Site-directed mutagenesis studies identified a number of residues important to the binding of phorbol esters to PKC. Analysis of the molecular modeling and mutagenesis results allows the development of a binding model for PKC ligands for which the precise binding nature is defined. The calculated hydrogen bond energies between the protein and various ligands in this binding model are consistent with their measured binding affinities. The binding site for phorbol esters and DAGs is located in a highly conserved, hydrophobic loop region formed by residues 6-12 and 20-27. For the binding elements in phorbol esters, the oxygen at C20 contributes most to the overall binding energy, and that at C3 plays a significant role. The oxygen atom at C12 is not directly involved in the interaction between phorbol esters and PKC. Our results also suggest that the oxygens at C9 and C13 are involved in PKC binding, while the oxygen at C4 is of minimal significance. These results are consistent with known structure-activity relationships in the phorbol ester family of compounds. Comparisons with the X-ray structure showed that although the X-ray data support the results for oxygens at C3, C12, and C20 of phorbol esters, they suggest different roles for oxygens at C4, C9, and C13. Several factors which may contribute to these discrepancies are discussed." @default.
- W1993211567 created "2016-06-24" @default.
- W1993211567 creator A5007763690 @default.
- W1993211567 creator A5010714518 @default.
- W1993211567 creator A5014617463 @default.
- W1993211567 creator A5029548499 @default.
- W1993211567 creator A5073754378 @default.
- W1993211567 date "1996-01-01" @default.
- W1993211567 modified "2023-10-18" @default.
- W1993211567 title "Molecular Modeling and Site-Directed Mutagenesis Studies of a Phorbol Ester-Binding Site in Protein Kinase C" @default.
- W1993211567 cites W1551547664 @default.
- W1993211567 cites W1573258139 @default.
- W1993211567 cites W1964839077 @default.
- W1993211567 cites W1973824969 @default.
- W1993211567 cites W1980104841 @default.
- W1993211567 cites W1993282564 @default.
- W1993211567 cites W1994920551 @default.
- W1993211567 cites W2001692359 @default.
- W1993211567 cites W2033157884 @default.
- W1993211567 cites W2033351557 @default.
- W1993211567 cites W2052500285 @default.
- W1993211567 cites W2076488165 @default.
- W1993211567 cites W2076756095 @default.
- W1993211567 cites W2084045483 @default.
- W1993211567 cites W2107718649 @default.
- W1993211567 cites W2136189765 @default.
- W1993211567 cites W2137882621 @default.
- W1993211567 cites W2162166182 @default.
- W1993211567 doi "https://doi.org/10.1021/jm950403n" @default.
- W1993211567 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8691452" @default.
- W1993211567 hasPublicationYear "1996" @default.
- W1993211567 type Work @default.
- W1993211567 sameAs 1993211567 @default.
- W1993211567 citedByCount "33" @default.
- W1993211567 countsByYear W19932115672012 @default.
- W1993211567 countsByYear W19932115672016 @default.
- W1993211567 countsByYear W19932115672018 @default.
- W1993211567 countsByYear W19932115672021 @default.
- W1993211567 crossrefType "journal-article" @default.
- W1993211567 hasAuthorship W1993211567A5007763690 @default.
- W1993211567 hasAuthorship W1993211567A5010714518 @default.
- W1993211567 hasAuthorship W1993211567A5014617463 @default.
- W1993211567 hasAuthorship W1993211567A5029548499 @default.
- W1993211567 hasAuthorship W1993211567A5073754378 @default.
- W1993211567 hasConcept C103408237 @default.
- W1993211567 hasConcept C104317684 @default.
- W1993211567 hasConcept C107824862 @default.
- W1993211567 hasConcept C143065580 @default.
- W1993211567 hasConcept C16318435 @default.
- W1993211567 hasConcept C178910836 @default.
- W1993211567 hasConcept C181199279 @default.
- W1993211567 hasConcept C185592680 @default.
- W1993211567 hasConcept C195794163 @default.
- W1993211567 hasConcept C2780043322 @default.
- W1993211567 hasConcept C41183919 @default.
- W1993211567 hasConcept C501734568 @default.
- W1993211567 hasConcept C55493867 @default.
- W1993211567 hasConcept C71240020 @default.
- W1993211567 hasConceptScore W1993211567C103408237 @default.
- W1993211567 hasConceptScore W1993211567C104317684 @default.
- W1993211567 hasConceptScore W1993211567C107824862 @default.
- W1993211567 hasConceptScore W1993211567C143065580 @default.
- W1993211567 hasConceptScore W1993211567C16318435 @default.
- W1993211567 hasConceptScore W1993211567C178910836 @default.
- W1993211567 hasConceptScore W1993211567C181199279 @default.
- W1993211567 hasConceptScore W1993211567C185592680 @default.
- W1993211567 hasConceptScore W1993211567C195794163 @default.
- W1993211567 hasConceptScore W1993211567C2780043322 @default.
- W1993211567 hasConceptScore W1993211567C41183919 @default.
- W1993211567 hasConceptScore W1993211567C501734568 @default.
- W1993211567 hasConceptScore W1993211567C55493867 @default.
- W1993211567 hasConceptScore W1993211567C71240020 @default.
- W1993211567 hasIssue "13" @default.
- W1993211567 hasLocation W19932115671 @default.
- W1993211567 hasLocation W19932115672 @default.
- W1993211567 hasOpenAccess W1993211567 @default.
- W1993211567 hasPrimaryLocation W19932115671 @default.
- W1993211567 hasRelatedWork W1597156601 @default.
- W1993211567 hasRelatedWork W1964949062 @default.
- W1993211567 hasRelatedWork W1974993927 @default.
- W1993211567 hasRelatedWork W1993211567 @default.
- W1993211567 hasRelatedWork W1995916976 @default.
- W1993211567 hasRelatedWork W1996923309 @default.
- W1993211567 hasRelatedWork W1999873842 @default.
- W1993211567 hasRelatedWork W2017218857 @default.
- W1993211567 hasRelatedWork W2062629157 @default.
- W1993211567 hasRelatedWork W241351009 @default.
- W1993211567 hasVolume "39" @default.
- W1993211567 isParatext "false" @default.
- W1993211567 isRetracted "false" @default.
- W1993211567 magId "1993211567" @default.
- W1993211567 workType "article" @default.