FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993224596> ?p ?o ?g. }

• W1993224596 endingPage "894" @default.
• W1993224596 startingPage "884" @default.
• W1993224596 abstract "The ability to analyze multiple polymorphic/mutation sites rapidly and accurately is pivotal in all areas of genetic analysis. We have applied single nucleotide primer extension (SNE) for detection of multiple point mutations in a micro-array format using two-color, fluorescent dye-tagged dideoxynucleoside triphosphate terminators (ddNTPs). The oligonucleotide primer ending one nucleotide short of the mutation site being probed is bound to the slide and single-base extended in place with two different Cy5/Cy3 dye-tagged terminators using solution-phase, locus-specific, single-stranded complementary templates generated by PCR from genomic DNA. The composite fluorescence produced contains peaks of distinct wave lengths corresponding to each Cy dye-tagged terminator incorporated, resulting in a fluorescent ‘fingerprint’ for each DNA target. DNA polymerase-catalyzed incorporation of Cy dye-tagged dideoxynucleoside triphosphates was dependent on the particular dyes, the specific ddNTP, the DNA target concentration, sequence of the template, on-slide temperature cycling and washing conditions. Results from analysis of mutations in the human hemochromatosis and connexin 26 genes show that this approach has several advantages over existing methods and is simple, rapid, robust, cost effective and accurate with potential applications in many areas of genetic analysis." @default.
• W1993224596 created "2016-06-24" @default.
• W1993224596 creator A5037083924 @default.
• W1993224596 creator A5038834229 @default.
• W1993224596 creator A5044277099 @default.
• W1993224596 creator A5048790207 @default.
• W1993224596 creator A5062228025 @default.
• W1993224596 creator A5076751469 @default.
• W1993224596 creator A5077301516 @default.
• W1993224596 creator A5082480194 @default.
• W1993224596 creator A5089328299 @default.
• W1993224596 date "2000-10-31" @default.
• W1993224596 modified "2023-10-11" @default.
• W1993224596 title "Simple two-color array-based approach for mutation detection" @default.
• W1993224596 cites W1562221129 @default.
• W1993224596 cites W1589411629 @default.
• W1993224596 cites W1925142648 @default.
• W1993224596 cites W1974960276 @default.
• W1993224596 cites W1978208924 @default.
• W1993224596 cites W1987739795 @default.
• W1993224596 cites W1991267989 @default.
• W1993224596 cites W1994718834 @default.
• W1993224596 cites W2001590540 @default.
• W1993224596 cites W2004667819 @default.
• W1993224596 cites W2008226460 @default.
• W1993224596 cites W2017589523 @default.
• W1993224596 cites W2026710381 @default.
• W1993224596 cites W2031928161 @default.
• W1993224596 cites W2032119463 @default.
• W1993224596 cites W2042443618 @default.
• W1993224596 cites W2049280274 @default.
• W1993224596 cites W2063005653 @default.
• W1993224596 cites W2082685728 @default.
• W1993224596 cites W2091619273 @default.
• W1993224596 cites W2100095605 @default.
• W1993224596 cites W2105025553 @default.
• W1993224596 cites W2107691596 @default.
• W1993224596 cites W2109056556 @default.
• W1993224596 cites W2116944848 @default.
• W1993224596 cites W2119166302 @default.
• W1993224596 cites W2127082697 @default.
• W1993224596 cites W2130974514 @default.
• W1993224596 cites W2137743001 @default.
• W1993224596 cites W2144345532 @default.
• W1993224596 cites W2156021383 @default.
• W1993224596 cites W2160524963 @default.
• W1993224596 cites W2162426408 @default.
• W1993224596 cites W2484973227 @default.
• W1993224596 cites W72492867 @default.
• W1993224596 doi "https://doi.org/10.1038/sj.ejhg.5200558" @default.
• W1993224596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11093279" @default.
• W1993224596 hasPublicationYear "2000" @default.
• W1993224596 type Work @default.
• W1993224596 sameAs 1993224596 @default.
• W1993224596 citedByCount "34" @default.
• W1993224596 countsByYear W19932245962013 @default.
• W1993224596 countsByYear W19932245962014 @default.
• W1993224596 countsByYear W19932245962015 @default.
• W1993224596 countsByYear W19932245962020 @default.
• W1993224596 crossrefType "journal-article" @default.
• W1993224596 hasAuthorship W1993224596A5037083924 @default.
• W1993224596 hasAuthorship W1993224596A5038834229 @default.
• W1993224596 hasAuthorship W1993224596A5044277099 @default.
• W1993224596 hasAuthorship W1993224596A5048790207 @default.
• W1993224596 hasAuthorship W1993224596A5062228025 @default.
• W1993224596 hasAuthorship W1993224596A5076751469 @default.
• W1993224596 hasAuthorship W1993224596A5077301516 @default.
• W1993224596 hasAuthorship W1993224596A5082480194 @default.
• W1993224596 hasAuthorship W1993224596A5089328299 @default.
• W1993224596 hasBestOaLocation W19932245961 @default.
• W1993224596 hasConcept C104317684 @default.
• W1993224596 hasConcept C116403925 @default.
• W1993224596 hasConcept C121332964 @default.
• W1993224596 hasConcept C1276947 @default.
• W1993224596 hasConcept C129312508 @default.
• W1993224596 hasConcept C13575015 @default.
• W1993224596 hasConcept C153911025 @default.
• W1993224596 hasConcept C176944494 @default.
• W1993224596 hasConcept C17757408 @default.
• W1993224596 hasConcept C178046730 @default.
• W1993224596 hasConcept C178790620 @default.
• W1993224596 hasConcept C185592680 @default.
• W1993224596 hasConcept C194857396 @default.
• W1993224596 hasConcept C2777563447 @default.
• W1993224596 hasConcept C3017666073 @default.
• W1993224596 hasConcept C501734568 @default.
• W1993224596 hasConcept C512185932 @default.
• W1993224596 hasConcept C54355233 @default.
• W1993224596 hasConcept C54887055 @default.
• W1993224596 hasConcept C552990157 @default.
• W1993224596 hasConcept C70721500 @default.
• W1993224596 hasConcept C86803240 @default.
• W1993224596 hasConceptScore W1993224596C104317684 @default.
• W1993224596 hasConceptScore W1993224596C116403925 @default.
• W1993224596 hasConceptScore W1993224596C121332964 @default.
• W1993224596 hasConceptScore W1993224596C1276947 @default.
• W1993224596 hasConceptScore W1993224596C129312508 @default.
• W1993224596 hasConceptScore W1993224596C13575015 @default.

• next