FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993233411> ?p ?o ?g. }

• W1993233411 endingPage "465" @default.
• W1993233411 startingPage "457" @default.
• W1993233411 abstract "It has been well demonstrated that interleukin-12 (IL-12) could be useful to defend against a variety of pathogens, to suppress tumor growth and metastasis, and even to be employed as an adjuvant of vaccines to enhance beneficial type 1 T helper (Th1) cell response over detrimental type 2 T helper (Th2) cell responses. To apply IL-12 genes in gene therapy such as a DNA vaccine, a pIL-12 vector was constructed that contained two cytomegalovirus (CMV) promoters to drive the expression of p35 and p40 subunits, respectively. In addition, a pscIL-12 vector was designed with a linker to fuse p35 cDNA with p40 cDNA to produce a single-chain IL-12 protein, ensuring not only that the expression of p35 and p40 subunits was equally expressed, but also that no free p40 subunits interfered with IL-12 activity. The data suggested pIL-12 could produce a rather high level of biologically active IL-12 after transfection of COS cell lines as well as C2C12 muscle cell lines, as measured by both concanavalin A blast proliferation assay and enzyme-linked immunosorbent assay. Interestingly, the pscIL-12 vector could also express a bioactive murine IL-12 fusion protein in vitro. Furthermore, in vivo functional studies also demonstrated that mice co-immunized with a pS vector expressing the major envelope protein of hepatitis B virus (HBV) and IL-12 vectors encoding native IL-12 or single-chain IL-12 fusion protein elicited higher levels of IgG2a anti-HBs antibody and of Th1-related cytokine. Because p35 and p40 genes can be expressed in a vector by using a single promoter, pscIL-12 should be useful in future applications for nucleic acid vaccination or for gene therapy against diseases." @default.
• W1993233411 created "2016-06-24" @default.
• W1993233411 creator A5008596479 @default.
• W1993233411 creator A5022404669 @default.
• W1993233411 creator A5044594740 @default.
• W1993233411 creator A5078795990 @default.
• W1993233411 date "1998-03-01" @default.
• W1993233411 modified "2023-09-27" @default.
• W1993233411 title "Construction of Vectors Expressing Bioactive Heterodimeric and Single-Chain Murine Interleukin-12 for Gene Therapy" @default.
• W1993233411 cites W1940408935 @default.
• W1993233411 cites W1970160145 @default.
• W1993233411 cites W1970279697 @default.
• W1993233411 cites W1977817262 @default.
• W1993233411 cites W1979514172 @default.
• W1993233411 cites W1988341070 @default.
• W1993233411 cites W1991587280 @default.
• W1993233411 cites W2006516536 @default.
• W1993233411 cites W2009179035 @default.
• W1993233411 cites W2023914649 @default.
• W1993233411 cites W2036101968 @default.
• W1993233411 cites W2056214993 @default.
• W1993233411 cites W2061517625 @default.
• W1993233411 cites W2069002836 @default.
• W1993233411 cites W2072200234 @default.
• W1993233411 cites W2073517578 @default.
• W1993233411 cites W2094315874 @default.
• W1993233411 cites W2095835949 @default.
• W1993233411 cites W2102156468 @default.
• W1993233411 cites W2104439753 @default.
• W1993233411 cites W2143209582 @default.
• W1993233411 cites W2156037313 @default.
• W1993233411 cites W4234332479 @default.
• W1993233411 cites W4250647025 @default.
• W1993233411 doi "https://doi.org/10.1089/hum.1998.9.4-457" @default.
• W1993233411 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9525307" @default.
• W1993233411 hasPublicationYear "1998" @default.
• W1993233411 type Work @default.
• W1993233411 sameAs 1993233411 @default.
• W1993233411 citedByCount "34" @default.
• W1993233411 countsByYear W19932334112013 @default.
• W1993233411 countsByYear W19932334112016 @default.
• W1993233411 countsByYear W19932334112017 @default.
• W1993233411 countsByYear W19932334112021 @default.
• W1993233411 countsByYear W19932334112022 @default.
• W1993233411 crossrefType "journal-article" @default.
• W1993233411 hasAuthorship W1993233411A5008596479 @default.
• W1993233411 hasAuthorship W1993233411A5022404669 @default.
• W1993233411 hasAuthorship W1993233411A5044594740 @default.
• W1993233411 hasAuthorship W1993233411A5078795990 @default.
• W1993233411 hasConcept C104317684 @default.
• W1993233411 hasConcept C111599444 @default.
• W1993233411 hasConcept C111829193 @default.
• W1993233411 hasConcept C123894998 @default.
• W1993233411 hasConcept C140704245 @default.
• W1993233411 hasConcept C144001008 @default.
• W1993233411 hasConcept C15152581 @default.
• W1993233411 hasConcept C153911025 @default.
• W1993233411 hasConcept C159047783 @default.
• W1993233411 hasConcept C187882448 @default.
• W1993233411 hasConcept C2522874641 @default.
• W1993233411 hasConcept C33161422 @default.
• W1993233411 hasConcept C40767141 @default.
• W1993233411 hasConcept C54009773 @default.
• W1993233411 hasConcept C55493867 @default.
• W1993233411 hasConcept C86803240 @default.
• W1993233411 hasConceptScore W1993233411C104317684 @default.
• W1993233411 hasConceptScore W1993233411C111599444 @default.
• W1993233411 hasConceptScore W1993233411C111829193 @default.
• W1993233411 hasConceptScore W1993233411C123894998 @default.
• W1993233411 hasConceptScore W1993233411C140704245 @default.
• W1993233411 hasConceptScore W1993233411C144001008 @default.
• W1993233411 hasConceptScore W1993233411C15152581 @default.
• W1993233411 hasConceptScore W1993233411C153911025 @default.
• W1993233411 hasConceptScore W1993233411C159047783 @default.
• W1993233411 hasConceptScore W1993233411C187882448 @default.
• W1993233411 hasConceptScore W1993233411C2522874641 @default.
• W1993233411 hasConceptScore W1993233411C33161422 @default.
• W1993233411 hasConceptScore W1993233411C40767141 @default.
• W1993233411 hasConceptScore W1993233411C54009773 @default.
• W1993233411 hasConceptScore W1993233411C55493867 @default.
• W1993233411 hasConceptScore W1993233411C86803240 @default.
• W1993233411 hasIssue "4" @default.
• W1993233411 hasLocation W19932334111 @default.
• W1993233411 hasLocation W19932334112 @default.
• W1993233411 hasOpenAccess W1993233411 @default.
• W1993233411 hasPrimaryLocation W19932334111 @default.
• W1993233411 hasRelatedWork W2262492967 @default.
• W1993233411 hasRelatedWork W2349536219 @default.
• W1993233411 hasRelatedWork W2350052461 @default.
• W1993233411 hasRelatedWork W2358891865 @default.
• W1993233411 hasRelatedWork W2364513136 @default.
• W1993233411 hasRelatedWork W2367085638 @default.
• W1993233411 hasRelatedWork W2372161277 @default.
• W1993233411 hasRelatedWork W2389269354 @default.
• W1993233411 hasRelatedWork W3031625311 @default.
• W1993233411 hasRelatedWork W33738171 @default.
• W1993233411 hasVolume "9" @default.
• W1993233411 isParatext "false" @default.

• next