Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993245715> ?p ?o ?g. }
- W1993245715 endingPage "746" @default.
- W1993245715 startingPage "738" @default.
- W1993245715 abstract "Abstract Ethanol is a tumor promoter and may promote metastasis of breast cancer. However, the underlying cellular/molecular mechanisms remain unknown. Overexpression and high activity of matrix metalloproteinase‐2 (MMP‐2) are frequently associated with metastatic breast cancers and serve as a prognostic indicator of clinical outcome. MMP‐2 is predominantly expressed in stromal fibroblasts and plays a pivotal role in regulating the invasive behavior of breast tumor cells. We hypothesized that ethanol may enhance the invasion of breast tumor cells by modulating the activity of fibroblastic MMP‐2. With in vitro models (HS68 and CCD1056SK human fibroblasts), we showed that ethanol at physiologically relevant concentrations (50–200 mg/dl) activated MMP‐2; conversely, at a higher concentration (400 mg/dl), it inhibited the MMP‐2 activity. Consistently, conditioned medium collected from ethanol (50–200 mg/dl)‐exposed fibroblasts markedly enhanced the invasive potential of breast cancer cells and mammary epithelial cells overexpressing ErbB2/HER2 (BT474, SKBR‐3 and HB2 ErbB2 cells) but had little effect on cells with low ErbB2 levels (BT20 and HB2 cells). In contrast, conditioned medium obtained from ethanol (400 mg/dl)‐treated fibroblasts inhibited cell invasion. Selective inhibitors of MMP‐2 (SB‐3CT and OA‐Hy) eliminated ethanol‐stimulated invasion, indicating that the effect of ethanol was mediated by MMP‐2. Ethanol activated conventional PKCs and JNKs in fibroblasts; inhibitors of PKC (Go6850 and Go6976) and JNKs (SP600125) significantly inhibited ethanol‐mediated MMP‐2 activation as well as cell invasion, indicating that PKCs and JNKs play a role in ethanol‐induced MMP‐2 activation and cell invasion in vitro . Thus, ethanol‐promoted breast cancer cell invasion may be mediated by the modulation of fibroblastic MMP‐2. © 2004 Wiley‐Liss, Inc." @default.
- W1993245715 created "2016-06-24" @default.
- W1993245715 creator A5020033545 @default.
- W1993245715 creator A5022290728 @default.
- W1993245715 creator A5041424398 @default.
- W1993245715 creator A5069253556 @default.
- W1993245715 creator A5071345446 @default.
- W1993245715 creator A5081063378 @default.
- W1993245715 date "2004-07-15" @default.
- W1993245715 modified "2023-10-14" @default.
- W1993245715 title "Ethanol-inducedin vitro invasion of breast cancer cells: The contribution of MMP-2 by fibroblasts" @default.
- W1993245715 cites W1567164810 @default.
- W1993245715 cites W1580481842 @default.
- W1993245715 cites W1605046409 @default.
- W1993245715 cites W1641731911 @default.
- W1993245715 cites W1648100608 @default.
- W1993245715 cites W169514863 @default.
- W1993245715 cites W1755902553 @default.
- W1993245715 cites W1765183309 @default.
- W1993245715 cites W1808822296 @default.
- W1993245715 cites W1902352259 @default.
- W1993245715 cites W1915020749 @default.
- W1993245715 cites W1964321121 @default.
- W1993245715 cites W1966160772 @default.
- W1993245715 cites W1967841383 @default.
- W1993245715 cites W1970446746 @default.
- W1993245715 cites W1972848761 @default.
- W1993245715 cites W1973546059 @default.
- W1993245715 cites W1980390662 @default.
- W1993245715 cites W1983219998 @default.
- W1993245715 cites W1983609115 @default.
- W1993245715 cites W1986884128 @default.
- W1993245715 cites W1990165115 @default.
- W1993245715 cites W2003797434 @default.
- W1993245715 cites W2008913484 @default.
- W1993245715 cites W2018211174 @default.
- W1993245715 cites W2021173111 @default.
- W1993245715 cites W2026566270 @default.
- W1993245715 cites W2031211419 @default.
- W1993245715 cites W2033083189 @default.
- W1993245715 cites W2036752075 @default.
- W1993245715 cites W2037858183 @default.
- W1993245715 cites W2038303988 @default.
- W1993245715 cites W2038828477 @default.
- W1993245715 cites W2043254359 @default.
- W1993245715 cites W2043810400 @default.
- W1993245715 cites W2046272184 @default.
- W1993245715 cites W2052538220 @default.
- W1993245715 cites W2052551989 @default.
- W1993245715 cites W2058473863 @default.
- W1993245715 cites W2059287943 @default.
- W1993245715 cites W2059910197 @default.
- W1993245715 cites W2062349124 @default.
- W1993245715 cites W2063355510 @default.
- W1993245715 cites W2067907963 @default.
- W1993245715 cites W2070852249 @default.
- W1993245715 cites W2071507111 @default.
- W1993245715 cites W2071708414 @default.
- W1993245715 cites W2076204015 @default.
- W1993245715 cites W2078698366 @default.
- W1993245715 cites W2081344406 @default.
- W1993245715 cites W2082087239 @default.
- W1993245715 cites W2084903524 @default.
- W1993245715 cites W2086170810 @default.
- W1993245715 cites W2088422531 @default.
- W1993245715 cites W2089313156 @default.
- W1993245715 cites W2092173590 @default.
- W1993245715 cites W2109084180 @default.
- W1993245715 cites W2117883091 @default.
- W1993245715 cites W2120811564 @default.
- W1993245715 cites W2123449729 @default.
- W1993245715 cites W2138365635 @default.
- W1993245715 cites W2149456035 @default.
- W1993245715 cites W2152803233 @default.
- W1993245715 cites W2157373954 @default.
- W1993245715 cites W2162639406 @default.
- W1993245715 cites W2188253534 @default.
- W1993245715 cites W2340940641 @default.
- W1993245715 cites W4246233276 @default.
- W1993245715 doi "https://doi.org/10.1002/ijc.20497" @default.
- W1993245715 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15386367" @default.
- W1993245715 hasPublicationYear "2004" @default.
- W1993245715 type Work @default.
- W1993245715 sameAs 1993245715 @default.
- W1993245715 citedByCount "50" @default.
- W1993245715 countsByYear W19932457152012 @default.
- W1993245715 countsByYear W19932457152013 @default.
- W1993245715 countsByYear W19932457152015 @default.
- W1993245715 countsByYear W19932457152016 @default.
- W1993245715 countsByYear W19932457152017 @default.
- W1993245715 countsByYear W19932457152018 @default.
- W1993245715 countsByYear W19932457152019 @default.
- W1993245715 countsByYear W19932457152020 @default.
- W1993245715 countsByYear W19932457152022 @default.
- W1993245715 crossrefType "journal-article" @default.
- W1993245715 hasAuthorship W1993245715A5020033545 @default.
- W1993245715 hasAuthorship W1993245715A5022290728 @default.
- W1993245715 hasAuthorship W1993245715A5041424398 @default.