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• W1993272007 abstract "A method for simultaneous and referenced 2D mapping of pH and pO2 is described. The experimental setup combines a fast gateable CCD camera as detector, a LED as excitation light source and a single-layer sensor membrane as optical transducer. The planar optode comprises a lipophilic fluorescein derivative (lifetime τ ∼ 5 ns) and platinum(II) mesotetrakis(pentafluorophenyl)porphyrin (τ ∼ 70 μs in the absence of a quencher) immobilized in a hydrogel matrix. Depending on the fluorescent pH indicator, a pH transition in the physiological range (pH 6−pH 8) or in the near-basic region (pH 7−pH 9) can be achieved. The measuring scheme involves the time-resolved acquisition of images in three windows during a series of square-shaped excitation pulses. A method allowing the calculation of both parameters from these three images is presented. The pH/pO2 hybrid sensor incorporating the pH indicator 2‘,7‘-dihexyl-5(6)-N-octadecyl-carboxamidofluorescein was characterized in detail. The pH and pO2 were determined with a maximum deviation of 0.03 pH unit and 6.5 hPa pO2, respectively, within the range of pH 7.6−pH 8.7 and 0−200 hPa pO2 in test measurements. The ionic strength (IS) cross-sensitivity was found to be relatively small (ΔpH/ΔIS < 3.5 × 10-4 mM-1 and ΔpO2/ΔIS <−0.053 hPa mM-1 at a transition from 0.5 to 0.1 M IS). Whereas a strong temperature effect on the sensor signal was observed (ΔpH/ΔT = 0.011−0.034 K-1 and ΔpO2/ΔT = 1.85−7.17 hPa K-1 in the range from 277 to 308 K). Examples of pH/pO2 images obtained in natural marine sediment are presented." @default.
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• W1993272007 date "2006-11-24" @default.
• W1993272007 modified "2023-10-17" @default.
• W1993272007 title "Time-Resolved pH/pO₂ Mapping with Luminescent Hybrid Sensors" @default.
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• W1993272007 doi "https://doi.org/10.1021/ac0606047" @default.
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