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- W1993289322 abstract "We report here the construction of a promoter-probe vector, pRS2, which can be utilized in either Acetobacter methanolicus MB 58 or Escherichia coli due to the presence of broad-host-range replicon RSF 1010. The vector provides several unique restriction sites for promoter cloning as well as resistance markers for the selection of transformants. The promoter-probe vector was constructed by inserting an EcoRI-SalI-polylinker fragment of pUC 19 into EcoRI/SalI digested pMK 16. The resulting plasmid, pRS1, was cloned into the unique EcoRI site of the broad-host-range plasmid RSF 1010. The vector was used to clone promoter-containing sequences derived from the A. methanolicus MB 58 chromosome as well as the E. coli lac-promoter." @default.
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- W1993289322 date "1989-01-01" @default.
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- W1993289322 title "Construction of a promoter-probe vector for the methanol-utilizing bacterium acetobacter methanolicus MB 58" @default.
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- W1993289322 doi "https://doi.org/10.1002/abio.370090306" @default.
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