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• W1993313586 abstract "ABSTRACT In the present study, we developed a cytotoxic T lymphocyte (CTL) epitope minigene-transduced dendritic cell (DC)-based vaccine against Listeria monocytogenes . Murine bone marrow-derived DCs were retrovirally transduced with a minigene for listeriolysin O (LLO) 91-99, a dominant CTL epitope of L. monocytogenes , and were injected into BALB/c mice intravenously. We found that the DC vaccine was capable of generating peptide-specific CD8 + T cells exhibiting LLO 91-99-specific cytotoxic activity and gamma interferon production, leading to induction of protective immunity to the bacterium. Furthermore, we demonstrated that the retrovirally transduced DC vaccine was more effective than a CTL epitope peptide-pulsed DC vaccine and a minigene DNA vaccine for eliciting antilisterial immunity. These results provide an alternative strategy in which retrovirally transduced DCs are used to design vaccines against intracellular pathogens." @default.
• W1993313586 created "2016-06-24" @default.
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• W1993313586 date "2003-04-01" @default.
• W1993313586 modified "2023-10-18" @default.
• W1993313586 title "Induction of Protective Immunity to Listeria monocytogenes with Dendritic Cells Retrovirally Transduced with a Cytotoxic T Lymphocyte Epitope Minigene" @default.
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• W1993313586 doi "https://doi.org/10.1128/iai.71.4.1748-1754.2003" @default.
• W1993313586 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/152038" @default.
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