Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993318371> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1993318371 endingPage "525" @default.
- W1993318371 startingPage "517" @default.
- W1993318371 abstract "DNA derived from the 5' spacers of the rRNA genes from Tetrahymena has unusual electrophoretic properties. These properties made it possible to devise a simple electrophoretic procedure for isolating specific rDNA spacer fragments from preparations of total nuclear DNA, enabling us to study DNA modifications at the level of unfractionated nuclei. We have employed the method to study the distribution of topoisomerase I binding sites on the r-chromatin (ribosomal chromatin) of Tetrahymena at the DNA sequence level. The presence of topoisomerase I in situ was detected by its ability to introduce single-strand cleavages into DNA. The positions of the cleavages were determined on DNA sequencing gels after isolation of the fragments. Topoisomerase I binding in r-chromatin is sequence specific and cleavage is confined to a 16 base-pair conserved sequence element previously determined to be a high-affinity binding site for topoisomerase I in vitro. The high degree of sequence specificity may be of important functional significance, as we find a similar sequence specificity with enzymes isolated from five evolutionarily distant species, indicating that preference for the 16 base-pair element is an intrinsic property of eukaryotic type I topoisomerases." @default.
- W1993318371 created "2016-06-24" @default.
- W1993318371 creator A5005262129 @default.
- W1993318371 creator A5017254416 @default.
- W1993318371 creator A5042563060 @default.
- W1993318371 date "1987-02-01" @default.
- W1993318371 modified "2023-09-23" @default.
- W1993318371 title "Mapping of sequence-specific chromatin proteins by a novel method: Topoisomerase I on Tetrahymena ribosomal chromatin" @default.
- W1993318371 cites W1510467852 @default.
- W1993318371 cites W1523173873 @default.
- W1993318371 cites W1546746814 @default.
- W1993318371 cites W1558516755 @default.
- W1993318371 cites W1577074552 @default.
- W1993318371 cites W1591207989 @default.
- W1993318371 cites W1963658914 @default.
- W1993318371 cites W1976366663 @default.
- W1993318371 cites W1993802920 @default.
- W1993318371 cites W1994272135 @default.
- W1993318371 cites W1996497833 @default.
- W1993318371 cites W1998394617 @default.
- W1993318371 cites W1999182662 @default.
- W1993318371 cites W2002043627 @default.
- W1993318371 cites W2023898023 @default.
- W1993318371 cites W2024916212 @default.
- W1993318371 cites W2028202441 @default.
- W1993318371 cites W2050256827 @default.
- W1993318371 cites W2056382700 @default.
- W1993318371 cites W2060093514 @default.
- W1993318371 cites W2082532569 @default.
- W1993318371 cites W2086099214 @default.
- W1993318371 cites W2087036937 @default.
- W1993318371 cites W2089017680 @default.
- W1993318371 cites W2093988305 @default.
- W1993318371 cites W2161095945 @default.
- W1993318371 cites W2168842935 @default.
- W1993318371 cites W38643767 @default.
- W1993318371 cites W43816515 @default.
- W1993318371 doi "https://doi.org/10.1016/0022-2836(87)90264-6" @default.
- W1993318371 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3035195" @default.
- W1993318371 hasPublicationYear "1987" @default.
- W1993318371 type Work @default.
- W1993318371 sameAs 1993318371 @default.
- W1993318371 citedByCount "49" @default.
- W1993318371 countsByYear W19933183712018 @default.
- W1993318371 countsByYear W19933183712021 @default.
- W1993318371 crossrefType "journal-article" @default.
- W1993318371 hasAuthorship W1993318371A5005262129 @default.
- W1993318371 hasAuthorship W1993318371A5017254416 @default.
- W1993318371 hasAuthorship W1993318371A5042563060 @default.
- W1993318371 hasConcept C104317684 @default.
- W1993318371 hasConcept C147897179 @default.
- W1993318371 hasConcept C153911025 @default.
- W1993318371 hasConcept C185581394 @default.
- W1993318371 hasConcept C2777166614 @default.
- W1993318371 hasConcept C54355233 @default.
- W1993318371 hasConcept C552990157 @default.
- W1993318371 hasConcept C55493867 @default.
- W1993318371 hasConcept C67905577 @default.
- W1993318371 hasConcept C83640560 @default.
- W1993318371 hasConcept C86803240 @default.
- W1993318371 hasConceptScore W1993318371C104317684 @default.
- W1993318371 hasConceptScore W1993318371C147897179 @default.
- W1993318371 hasConceptScore W1993318371C153911025 @default.
- W1993318371 hasConceptScore W1993318371C185581394 @default.
- W1993318371 hasConceptScore W1993318371C2777166614 @default.
- W1993318371 hasConceptScore W1993318371C54355233 @default.
- W1993318371 hasConceptScore W1993318371C552990157 @default.
- W1993318371 hasConceptScore W1993318371C55493867 @default.
- W1993318371 hasConceptScore W1993318371C67905577 @default.
- W1993318371 hasConceptScore W1993318371C83640560 @default.
- W1993318371 hasConceptScore W1993318371C86803240 @default.
- W1993318371 hasIssue "3" @default.
- W1993318371 hasLocation W19933183711 @default.
- W1993318371 hasLocation W19933183712 @default.
- W1993318371 hasOpenAccess W1993318371 @default.
- W1993318371 hasPrimaryLocation W19933183711 @default.
- W1993318371 hasRelatedWork W1920751942 @default.
- W1993318371 hasRelatedWork W1979566739 @default.
- W1993318371 hasRelatedWork W1992884065 @default.
- W1993318371 hasRelatedWork W2024441663 @default.
- W1993318371 hasRelatedWork W2058763582 @default.
- W1993318371 hasRelatedWork W2099924252 @default.
- W1993318371 hasRelatedWork W2112354301 @default.
- W1993318371 hasRelatedWork W2400545929 @default.
- W1993318371 hasRelatedWork W2406423740 @default.
- W1993318371 hasRelatedWork W2468901029 @default.
- W1993318371 hasVolume "193" @default.
- W1993318371 isParatext "false" @default.
- W1993318371 isRetracted "false" @default.
- W1993318371 magId "1993318371" @default.
- W1993318371 workType "article" @default.