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• W1993332017 abstract "We present a convenient method for determining free or non-protein-bound iron in biological fluids. The new method is based on the bathophenantroline method for determination of total serum iron, and comprises binding of iron by a chromogenic chelator (bathophenantroline-disulphonate, BPS), which is specific for ferrous iron. The ferrous complex of BPS absorbs strongly at 535 nm, and the detection limit is less than 1 microM in a sample size of 50 microliters. The chelator does not liberate iron from either haemoglobin or transferrin. Interference from copper or zinc in concentrations up to 50 microM does not significantly disturb measurements. The main problem when measuring in blood plasma, the high and fluctuating background in the region around 535 nm, has been overcome through filtering techniques. Data from measurements of ferrous iron in microdialysate, cerebrospinal fluid, and blood plasma in different animal models and clinical conditions are presented as illustrative examples of the usefulness of the method. The method allows the determination of ferric, as well as ferrous, iron in the same sample." @default.
• W1993332017 created "2016-06-24" @default.
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• W1993332017 date "2002-01-01" @default.
• W1993332017 modified "2023-10-12" @default.
• W1993332017 title "A Simple and Rapid Method for the Determination of free Iron in Biological Fluids" @default.
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• W1993332017 doi "https://doi.org/10.1080/10715760290029128" @default.
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