Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993350879> ?p ?o ?g. }
- W1993350879 endingPage "1851" @default.
- W1993350879 startingPage "1835" @default.
- W1993350879 abstract "Adaptor protein complexes (AP) are major components of the cytoplasmic coat found on clathrin-coated vesicles. Here, we report the molecular and functional characterization of Dictyostelium clathrin-associated AP-1 complex, which in mammalian cells, participates mainly in budding of clathrin-coated vesicles from the trans-Golgi network (TGN). The γ-adaptin AP-1 subunit was cloned and shown to belong to a Golgi-localized 300-kDa protein complex. Time-lapse analysis of cells expressing γ-adaptin tagged with the green-fluorescent protein demonstrates the dynamics of AP-1–coated structures leaving the Golgi apparatus and rarely moving toward the TGN. Targeted disruption of the AP-1 medium chain results in viable cells displaying a severe growth defect and a delayed developmental cycle compared with parental cells. Lysosomal enzymes are constitutively secreted as precursors, suggesting that protein transport between the TGN and lysosomes is defective. Although endocytic protein markers are correctly localized to endosomal compartments, morphological and ultrastructural studies reveal the absence of large endosomal vacuoles and an increased number of small vacuoles. In addition, the function of the contractile vacuole complex (CV), an osmoregulatory organelle is impaired and some CV components are not correctly targeted." @default.
- W1993350879 created "2016-06-24" @default.
- W1993350879 creator A5003320334 @default.
- W1993350879 creator A5010323246 @default.
- W1993350879 creator A5032685463 @default.
- W1993350879 creator A5041049058 @default.
- W1993350879 creator A5042868602 @default.
- W1993350879 creator A5061079678 @default.
- W1993350879 creator A5061539239 @default.
- W1993350879 creator A5073269050 @default.
- W1993350879 creator A5074290277 @default.
- W1993350879 creator A5089183492 @default.
- W1993350879 date "2003-05-01" @default.
- W1993350879 modified "2023-10-18" @default.
- W1993350879 title "The AP-1 Clathrin-adaptor Is Required for Lysosomal Enzymes Sorting and Biogenesis of the Contractile Vacuole Complex in<i>Dictyostelium</i>Cells" @default.
- W1993350879 cites W1485689124 @default.
- W1993350879 cites W1497255723 @default.
- W1993350879 cites W1501427183 @default.
- W1993350879 cites W1501496398 @default.
- W1993350879 cites W1547198991 @default.
- W1993350879 cites W1550731887 @default.
- W1993350879 cites W1600119101 @default.
- W1993350879 cites W1959046849 @default.
- W1993350879 cites W1964174724 @default.
- W1993350879 cites W1972087498 @default.
- W1993350879 cites W1973845643 @default.
- W1993350879 cites W1977333395 @default.
- W1993350879 cites W1979513617 @default.
- W1993350879 cites W1979815209 @default.
- W1993350879 cites W1984310356 @default.
- W1993350879 cites W1984730682 @default.
- W1993350879 cites W1987672004 @default.
- W1993350879 cites W1993423963 @default.
- W1993350879 cites W1994266010 @default.
- W1993350879 cites W1994528127 @default.
- W1993350879 cites W1996121280 @default.
- W1993350879 cites W1997361579 @default.
- W1993350879 cites W1998974411 @default.
- W1993350879 cites W2006196178 @default.
- W1993350879 cites W2009942743 @default.
- W1993350879 cites W2012848672 @default.
- W1993350879 cites W2016724938 @default.
- W1993350879 cites W2019196882 @default.
- W1993350879 cites W2020269189 @default.
- W1993350879 cites W2027970091 @default.
- W1993350879 cites W2030222561 @default.
- W1993350879 cites W2033240631 @default.
- W1993350879 cites W2035477123 @default.
- W1993350879 cites W2044293736 @default.
- W1993350879 cites W2045388161 @default.
- W1993350879 cites W2045883887 @default.
- W1993350879 cites W2049766220 @default.
- W1993350879 cites W2051255398 @default.
- W1993350879 cites W2052531986 @default.
- W1993350879 cites W2055371745 @default.
- W1993350879 cites W2055920490 @default.
- W1993350879 cites W2059639250 @default.
- W1993350879 cites W2062145837 @default.
- W1993350879 cites W2063458187 @default.
- W1993350879 cites W2069120227 @default.
- W1993350879 cites W2070497054 @default.
- W1993350879 cites W2086284430 @default.
- W1993350879 cites W2086845337 @default.
- W1993350879 cites W2089209951 @default.
- W1993350879 cites W2093538950 @default.
- W1993350879 cites W2094104389 @default.
- W1993350879 cites W2100083548 @default.
- W1993350879 cites W2103481532 @default.
- W1993350879 cites W2104063456 @default.
- W1993350879 cites W2104433470 @default.
- W1993350879 cites W2109240208 @default.
- W1993350879 cites W2109518433 @default.
- W1993350879 cites W2114410573 @default.
- W1993350879 cites W2116365110 @default.
- W1993350879 cites W2116537957 @default.
- W1993350879 cites W2117352422 @default.
- W1993350879 cites W2119260049 @default.
- W1993350879 cites W2120140317 @default.
- W1993350879 cites W2123743297 @default.
- W1993350879 cites W2133425292 @default.
- W1993350879 cites W2133698151 @default.
- W1993350879 cites W2134344512 @default.
- W1993350879 cites W2137344375 @default.
- W1993350879 cites W2140243527 @default.
- W1993350879 cites W2143205348 @default.
- W1993350879 cites W2145089843 @default.
- W1993350879 cites W2158759493 @default.
- W1993350879 cites W2164253947 @default.
- W1993350879 cites W2166175048 @default.
- W1993350879 cites W2168205500 @default.
- W1993350879 cites W2185203631 @default.
- W1993350879 cites W2186496049 @default.
- W1993350879 cites W2419492482 @default.
- W1993350879 cites W2742272146 @default.
- W1993350879 doi "https://doi.org/10.1091/mbc.e02-10-0627" @default.
- W1993350879 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/165081" @default.
- W1993350879 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12802059" @default.
- W1993350879 hasPublicationYear "2003" @default.