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- W1993373785 abstract "Methods A methodology and a mathematical relationship have been developed that allow quantitation of the expression levels of cellular surface antigens, in terms of antibody binding capacities (ABC). This methodology uses immunomagnetically labeled cells and calibration microbeads combined with cell tracking velocimetry (CTV) technology to measure magnetophoretic mobilities corresponding to cellular ABC. The mobility measurements were accomplished by microscopically recording and calculating the velocity of immunomagnetically labeled QSC microbeads and cells in a nearly constant magnetic energy gradient. Results Transformed fibrosarcoma cells were given controlled treatments of interferon-α in order to manipulate CD2 antigen expression levels. These cells were then immunomagnetically labeled with anti-CD2 FITC antibodies and anti-FITC MACS paramagnetic nanoparticles. Measured magnetophoretic mobilities were used to calculate ABC for these cells, corresponding to CD2 expression levels. Conclusion The results from CTV and flow cytometry (FCM) qualitatively verify that these fibrosarcoma cells express elevated levels of CD2 molecules with increasing interferon-α treatment from 0 to 24 h. The mean basal CD2 expression level, in terms of ABC, was calculated to be 27,000 from CTV analysis, whereas FCM indicates a comparable ABC value of 33,000. Cytometry 44:137–147, 2001. © 2001 Wiley-Liss, Inc." @default.
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- W1993373785 date "2001-01-01" @default.
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- W1993373785 title "Measurement of CD2 expression levels of IFN-?-treated fibrosarcomas using cell tracking velocimetry" @default.
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- W1993373785 doi "https://doi.org/10.1002/1097-0320(20010601)44:2<137::aid-cyto1093>3.0.co;2-d" @default.
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