FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993382008> ?p ?o ?g. }

• W1993382008 endingPage "2453" @default.
• W1993382008 startingPage "2433" @default.
• W1993382008 abstract "An enzyme which catalyzes the addition of a single UMP residue from UTP to the 3'-end of an RNA primer and which is referred to as terminal uridylyl transferase (TUT) has been extensively purified from the membrane fraction of vigna unguiculata leaves. The purification procedure involved (i) solubilization by cation depletion (ii) DEAE-Sepharose CL-6B column chromatography (iii) affinity chromatography of poly(U)-Sepharose 4B and (iv) glycerol gradient centrifugation. The molecular weight of the native enzyme was approximately 50,000 as determined by velocity sedimentation. Under conditions that were optimal for UMP-incorporation (5 mM Mg2+, low salt, 30 degrees C) TUT displayed a marked specificity for UTP as substrate, was unable to incorporate deoxyribonucleoside triphosphates and required a single-stranded oligo- or polyribonucleotide as primer. When oligoA20, tRNAasp of E. coli or alfalfa mosaic virus RNA 4 were used as primers at various substrate to primer ratio's, the vast majority of the product appeared to consist of primer molecules elongated with a single UMP residue as shown by polyacrylamide gelelectrophoresis and nearest neighbour analysis. We believe TUT to be a novel enzyme which has not been reported before and which may be a feasible tool in RNA sequencing as it enables the specific 3'-terminal labeling of RNA molecules." @default.
• W1993382008 created "2016-06-24" @default.
• W1993382008 creator A5024397273 @default.
• W1993382008 creator A5049882111 @default.
• W1993382008 creator A5051817994 @default.
• W1993382008 creator A5077141538 @default.
• W1993382008 date "1981-01-01" @default.
• W1993382008 modified "2023-10-13" @default.
• W1993382008 title "Terminal uridylyl transferase of Vigna unguiculata: purification and characterization of an enzyme catalyzing the addition of a single UMP residue to the 3′-end of an RNA primer" @default.
• W1993382008 cites W1031323298 @default.
• W1993382008 cites W1488715900 @default.
• W1993382008 cites W1516648546 @default.
• W1993382008 cites W1524353567 @default.
• W1993382008 cites W1531980515 @default.
• W1993382008 cites W1536346720 @default.
• W1993382008 cites W1538191571 @default.
• W1993382008 cites W1611482599 @default.
• W1993382008 cites W1893651774 @default.
• W1993382008 cites W1978826574 @default.
• W1993382008 cites W1985032452 @default.
• W1993382008 cites W2003485143 @default.
• W1993382008 cites W2006669281 @default.
• W1993382008 cites W2031546477 @default.
• W1993382008 cites W2043974503 @default.
• W1993382008 cites W2054479556 @default.
• W1993382008 cites W2064962869 @default.
• W1993382008 cites W2069992210 @default.
• W1993382008 cites W2072386244 @default.
• W1993382008 cites W2082559634 @default.
• W1993382008 cites W2083854003 @default.
• W1993382008 cites W2086733743 @default.
• W1993382008 cites W2087053996 @default.
• W1993382008 cites W2087526610 @default.
• W1993382008 cites W2098485788 @default.
• W1993382008 cites W2128635872 @default.
• W1993382008 cites W2129224938 @default.
• W1993382008 cites W2165439491 @default.
• W1993382008 cites W2213213669 @default.
• W1993382008 cites W2418860382 @default.
• W1993382008 cites W2916651983 @default.
• W1993382008 cites W4210956896 @default.
• W1993382008 doi "https://doi.org/10.1093/nar/9.11.2433" @default.
• W1993382008 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/326863" @default.
• W1993382008 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6269049" @default.
• W1993382008 hasPublicationYear "1981" @default.
• W1993382008 type Work @default.
• W1993382008 sameAs 1993382008 @default.
• W1993382008 citedByCount "36" @default.
• W1993382008 crossrefType "journal-article" @default.
• W1993382008 hasAuthorship W1993382008A5024397273 @default.
• W1993382008 hasAuthorship W1993382008A5049882111 @default.
• W1993382008 hasAuthorship W1993382008A5051817994 @default.
• W1993382008 hasAuthorship W1993382008A5077141538 @default.
• W1993382008 hasBestOaLocation W19933820082 @default.
• W1993382008 hasConcept C104317684 @default.
• W1993382008 hasConcept C178790620 @default.
• W1993382008 hasConcept C179247698 @default.
• W1993382008 hasConcept C181199279 @default.
• W1993382008 hasConcept C185592680 @default.
• W1993382008 hasConcept C2777563447 @default.
• W1993382008 hasConcept C2779862049 @default.
• W1993382008 hasConcept C2779881004 @default.
• W1993382008 hasConcept C2781338088 @default.
• W1993382008 hasConcept C43617362 @default.
• W1993382008 hasConcept C512185932 @default.
• W1993382008 hasConcept C55493867 @default.
• W1993382008 hasConcept C6557445 @default.
• W1993382008 hasConcept C67705224 @default.
• W1993382008 hasConcept C86803240 @default.
• W1993382008 hasConceptScore W1993382008C104317684 @default.
• W1993382008 hasConceptScore W1993382008C178790620 @default.
• W1993382008 hasConceptScore W1993382008C179247698 @default.
• W1993382008 hasConceptScore W1993382008C181199279 @default.
• W1993382008 hasConceptScore W1993382008C185592680 @default.
• W1993382008 hasConceptScore W1993382008C2777563447 @default.
• W1993382008 hasConceptScore W1993382008C2779862049 @default.
• W1993382008 hasConceptScore W1993382008C2779881004 @default.
• W1993382008 hasConceptScore W1993382008C2781338088 @default.
• W1993382008 hasConceptScore W1993382008C43617362 @default.
• W1993382008 hasConceptScore W1993382008C512185932 @default.
• W1993382008 hasConceptScore W1993382008C55493867 @default.
• W1993382008 hasConceptScore W1993382008C6557445 @default.
• W1993382008 hasConceptScore W1993382008C67705224 @default.
• W1993382008 hasConceptScore W1993382008C86803240 @default.
• W1993382008 hasIssue "11" @default.
• W1993382008 hasLocation W19933820081 @default.
• W1993382008 hasLocation W19933820082 @default.
• W1993382008 hasLocation W19933820083 @default.
• W1993382008 hasLocation W19933820084 @default.
• W1993382008 hasOpenAccess W1993382008 @default.
• W1993382008 hasPrimaryLocation W19933820081 @default.
• W1993382008 hasRelatedWork W2004279953 @default.
• W1993382008 hasRelatedWork W2017193031 @default.
• W1993382008 hasRelatedWork W2021282793 @default.
• W1993382008 hasRelatedWork W2361580214 @default.
• W1993382008 hasRelatedWork W2369453125 @default.
• W1993382008 hasRelatedWork W2370189926 @default.
• W1993382008 hasRelatedWork W2373846342 @default.

• next