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- W1993410433 abstract "A novel amperometric bienzymatic biosensor has been developed based on the incorporation of Lactate Oxidase (LOx) and Horseradish Peroxidase (HRP) into a carbon nanotube/polysulfone membrane by the phase inversion technique onto screen-printed electrodes (SPEs). In order to improve the sensitivity and reduce the working potential, experimental conditions have been optimized and ferrocene has also been incorporated into the membrane as a redox mediator of the enzymatic reactions, which allows the reduction of H2O2 at −100 mV. Measurements were carried out in phosphate buffer solution at pH 7.5 and under batch conditions. The biosensor response time to L-lactate was only 20 s and showed a good reproducibility (RSD 2.7%). Moreover, the detection limit was 0.05 mg L−1 of L-lactate with a linear interval range from 0.1 mg L−1 to 5 mg L−1. Finally, the biosensor has been applied to the determination of L-lactic acid in different wine and beer samples. Then, the results obtained with the biosensor were compared with the ones obtained using, as a reference method, a commercial kit based on spectrophotometric measurements, obtaining an excellent agreement between the results, validating our approach." @default.
- W1993410433 created "2016-06-24" @default.
- W1993410433 creator A5055091953 @default.
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- W1993410433 date "2012-01-01" @default.
- W1993410433 modified "2023-10-18" @default.
- W1993410433 title "Amperometric bienzymatic biosensor for l-lactate analysis in wine and beer samples" @default.
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- W1993410433 doi "https://doi.org/10.1039/c2an35227c" @default.
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