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- W1993442631 abstract "Bifidobacterium bifidum var. pennsylvanicus acquires ferrous iron at a reasonable rate when this bacterium — in the resting state — is incubated with ferrous iron in either a modified Hanks' solution, ot Norris medium in which the bacteria had previously grown. Ferrous iron binding was substantially lower in unused Norris medium containing human milk whey, because it contained iron binding inhibitors that were degraded during bacterial growth. The inhibitors were identified as human milk whey components and bovine casein digest. The affinity of bacteria for ferrous iron was dependent on the iron concentration of the media. In modified Hanks' solution, the affinity of cells for iron at low iron concentrations was some 250-fold greater than that at high iron concentrations. Ferrous iron binding did not take place it a carbon source was omitted from the media or if the cells had previously been heated at 80°C for 20 min. Surface binding of ferrous iron to the bacterial membrane was minimal. Trypsin and microbial proteinase treatment of bacteria did not inhibit iron uptake, making an iron-specific receptor on bifidobacterial cell membranes unlikely. Iron binding by bifidobacteria is an energy-requiring process dependent upon the operation of a heat-sensitive enzyme machinery, and it does not involve an extracellular iron carrier." @default.
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- W1993442631 date "1986-10-01" @default.
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- W1993442631 title "The mechanism of ferrous iron binding by suspensions of Bifidobacterium bifidum var. pennsylvanicus" @default.
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- W1993442631 doi "https://doi.org/10.1016/0304-4165(86)90227-8" @default.
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