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- W1993459113 abstract "IR spectroscopic images were recorded using an Equinox Bruker FTIR spectrometer coupled to a Hyperion 3000 imaging system equipped with a mercury cadmium telluride (MCT)-based focal plane array (FPA) detector of 64×64 pixels (Bruker Optik, Ettlingen, Germany). Images containing 4096 IR spectra at 8 cm-1 spectral resolution were acquired by coadding 256 interferograms in about 5 minutes. All the data processing was carried out by the program “Kinetics” running under MatLab. The “Kinetics” software, previously used for FTIR spectrum processing and analysis, was extended for the processing of FPA-acquired FTIR data. Different types of images can be generated, either based on the absorbance at 1 wavenumber or the ratio of the absorbances at 2 wavenumbers or more advanced combination of the data. We show here that protein secondary structure content evaluated as described in [1] sheds light on the molecular determinants that allow the differentiation between sub-structures in tissues. Similarly we shall illustrate heterogeneity in prostate cancer cells (PC-3) in culture based on secondary structure imaging. References [1] Goormaghtigh,E., J.M.Ruysschaert, and V.Raussens. Evaluation of the information content in infrared spectra for protein secondary structure determination, Biophys.J. 90 (2006) 2946-2957." @default.
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- W1993459113 date "2010-01-01" @default.
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- W1993459113 title "Data Processing in FTIR Imaging of Cells and Tissues: Towards Protein Secondary Structure Imaging" @default.
- W1993459113 doi "https://doi.org/10.1016/j.bpj.2009.12.4070" @default.
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