Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993526634> ?p ?o ?g. }
- W1993526634 endingPage "388" @default.
- W1993526634 startingPage "375" @default.
- W1993526634 abstract "Fast-growing mycobacteria are considered essential members of the polycyclic aromatic hydrocarbons (PAH) degrading bacterial community in PAH-contaminated soils. To study the natural role and diversity of the Mycobacterium community in contaminated soils, a culture-independent fingerprinting method based on PCR combined with denaturing gradient gel electrophoresis (DGGE) was developed. New PCR primers were selected which specifically targeted the 16S rRNA genes of fast-growing mycobacteria, and single-band DGGE profiles of amplicons were obtained for most Mycobacterium strains tested. Strains belonging to the same species revealed identical DGGE fingerprints, and in most cases, but not all, these fingerprints were typical for one species, allowing partial differentiation between species in a Mycobacterium community. Mycobacterium strains inoculated in soil were detected with a detection limit of 106 CFU g−1 of soil using the new primer set as such, or approximately 102 CFU g−1 in a nested PCR approach combining eubacterial and the Mycobacterium specific primers. Using the PCR-DGGE method, different species could be individually recognized in a mixed Mycobacterium community. This approach was used to rapidly assess the Mycobacterium community structure of several PAH-contaminated soils of diverse origin with different overall contamination profiles, pollution concentrations and chemical-physical soil characteristics. In the non-contaminated soil, most of the recovered 16SrRNA gene sequence did not match with previous described PAH-degrading Mycobacterium strains. In most PAH-contaminated soils, mycobacteria were detected which were closely related to fast-growing species such as Mycobacterium frederiksbergense and Mycobacterium austroafricanum, species that are known to include strains with PAH-degrading capacities. Interestingly, 16S rRNA genes related to M. tusciae sequences, a Mycobacterium species so far not reported in relation to biodegradation of PAHs, were detected in all contaminated soils." @default.
- W1993526634 created "2016-06-24" @default.
- W1993526634 creator A5000462692 @default.
- W1993526634 creator A5020835723 @default.
- W1993526634 creator A5034405463 @default.
- W1993526634 creator A5052218386 @default.
- W1993526634 creator A5078760152 @default.
- W1993526634 creator A5081994433 @default.
- W1993526634 creator A5090222404 @default.
- W1993526634 date "2005-02-01" @default.
- W1993526634 modified "2023-10-11" @default.
- W1993526634 title "Occurrence and community composition of fast-growing Mycobacterium in soils contaminated with polycyclic aromatic hydrocarbons" @default.
- W1993526634 cites W1548396112 @default.
- W1993526634 cites W1563247761 @default.
- W1993526634 cites W1602347636 @default.
- W1993526634 cites W1677926307 @default.
- W1993526634 cites W1761387917 @default.
- W1993526634 cites W1886356207 @default.
- W1993526634 cites W1909187058 @default.
- W1993526634 cites W1946614122 @default.
- W1993526634 cites W1963844781 @default.
- W1993526634 cites W1970472614 @default.
- W1993526634 cites W1977045240 @default.
- W1993526634 cites W1996423252 @default.
- W1993526634 cites W2034855430 @default.
- W1993526634 cites W2055043387 @default.
- W1993526634 cites W2072308015 @default.
- W1993526634 cites W2072412393 @default.
- W1993526634 cites W2078761998 @default.
- W1993526634 cites W2089972364 @default.
- W1993526634 cites W2098761708 @default.
- W1993526634 cites W2102424972 @default.
- W1993526634 cites W2102555068 @default.
- W1993526634 cites W2107543996 @default.
- W1993526634 cites W2109612651 @default.
- W1993526634 cites W2110271984 @default.
- W1993526634 cites W2111858111 @default.
- W1993526634 cites W2113355897 @default.
- W1993526634 cites W2117569420 @default.
- W1993526634 cites W2123815866 @default.
- W1993526634 cites W2124449782 @default.
- W1993526634 cites W2129360308 @default.
- W1993526634 cites W2130694174 @default.
- W1993526634 cites W2134683273 @default.
- W1993526634 cites W2141927954 @default.
- W1993526634 cites W2142374516 @default.
- W1993526634 cites W2142717936 @default.
- W1993526634 cites W2146447556 @default.
- W1993526634 cites W2153303660 @default.
- W1993526634 cites W2155776392 @default.
- W1993526634 cites W2156492633 @default.
- W1993526634 cites W2157900653 @default.
- W1993526634 cites W2161979494 @default.
- W1993526634 cites W2162578061 @default.
- W1993526634 cites W2163014180 @default.
- W1993526634 cites W2164091046 @default.
- W1993526634 cites W2169333662 @default.
- W1993526634 cites W2176371198 @default.
- W1993526634 cites W276370537 @default.
- W1993526634 cites W3022314104 @default.
- W1993526634 cites W4241391257 @default.
- W1993526634 cites W4242565687 @default.
- W1993526634 cites W4248176371 @default.
- W1993526634 cites W89731584 @default.
- W1993526634 doi "https://doi.org/10.1016/j.femsec.2004.09.015" @default.
- W1993526634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16329885" @default.
- W1993526634 hasPublicationYear "2005" @default.
- W1993526634 type Work @default.
- W1993526634 sameAs 1993526634 @default.
- W1993526634 citedByCount "95" @default.
- W1993526634 countsByYear W19935266342012 @default.
- W1993526634 countsByYear W19935266342013 @default.
- W1993526634 countsByYear W19935266342014 @default.
- W1993526634 countsByYear W19935266342015 @default.
- W1993526634 countsByYear W19935266342016 @default.
- W1993526634 countsByYear W19935266342017 @default.
- W1993526634 countsByYear W19935266342018 @default.
- W1993526634 countsByYear W19935266342019 @default.
- W1993526634 countsByYear W19935266342020 @default.
- W1993526634 countsByYear W19935266342021 @default.
- W1993526634 countsByYear W19935266342022 @default.
- W1993526634 countsByYear W19935266342023 @default.
- W1993526634 crossrefType "journal-article" @default.
- W1993526634 hasAuthorship W1993526634A5000462692 @default.
- W1993526634 hasAuthorship W1993526634A5020835723 @default.
- W1993526634 hasAuthorship W1993526634A5034405463 @default.
- W1993526634 hasAuthorship W1993526634A5052218386 @default.
- W1993526634 hasAuthorship W1993526634A5078760152 @default.
- W1993526634 hasAuthorship W1993526634A5081994433 @default.
- W1993526634 hasAuthorship W1993526634A5090222404 @default.
- W1993526634 hasBestOaLocation W19935266341 @default.
- W1993526634 hasConcept C104317684 @default.
- W1993526634 hasConcept C112570922 @default.
- W1993526634 hasConcept C172365310 @default.
- W1993526634 hasConcept C18903297 @default.
- W1993526634 hasConcept C198549448 @default.
- W1993526634 hasConcept C2780374374 @default.
- W1993526634 hasConcept C42062724 @default.