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- W1993564826 abstract "ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the specificity and sensitivity of such analysis, we developed a new technology called ChIP-DSL using the DNA selection and ligation (DSL) strategy, permitting robust analysis with much reduced materials compared with standard procedures. We profiled general and sequence-specific DNA binding transcription factors using a full human genome promoter array based on the ChIP-DSL technology, revealing an unprecedented number of the estrogen receptor (ERα) target genes in MCF-7 cells. Coupled with gene expression profiling, we found that only a fraction of these direct ERα target genes were highly responsive to estrogen and that the expression of those ERα-bound, estrogen-inducible genes was associated with breast cancer progression in humans. This study demonstrates the power of the ChIP-DSL technology in revealing regulatory gene expression programs that have been previously invisible in the human genome." @default.
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- W1993564826 date "2007-03-20" @default.
- W1993564826 modified "2023-10-17" @default.
- W1993564826 title "Sensitive ChIP-DSL technology reveals an extensive estrogen receptor α-binding program on human gene promoters" @default.
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- W1993564826 doi "https://doi.org/10.1073/pnas.0700715104" @default.
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