Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993575755> ?p ?o ?g. }
Showing items 1 to 74 of
74
with 100 items per page.
- W1993575755 endingPage "532" @default.
- W1993575755 startingPage "532" @default.
- W1993575755 abstract "Using a novel sequencing method, Harvard University researchers aim to reduce the time taken to sequence a genome to a matter of hours [Wang and Branton (2001) Nat. Biotechnol. 19, 622–623]. Sequencing the genome took the public consortium more than a decade, and the Celera biotechnology company approximately nine months (with input from the public project). The novel method, developed by Daniel Branton and Jene Golovchenko of Harvard University (Cambridge, MA, USA), together with David Deamer of the University of California (CA, USA), uses a lipid bilayer to compartmentalize a dish into two separate regions. A long single strand of (negatively charged) nucleotides is placed on one side of the bilayer. Application of α-hemolysin causes formation of a nanopore in the membrane, across which a voltage bias is then applied. The nucleotide chain worms its way through the channel towards the positive side of the dish, such that each nucleotide flowing through the nanopore generates a distinct current. The nucleotides can pass through the channel pore only in sequential, single-file order because the limiting diameter of the pore (∼1.5 nm) can accommodate only one strand of DNA or RNA at a time. Very small changes in the distribution of charges within the channel can give rise to very large changes in the ionic flux through the channel pore. The aim is that each nucleotide will be identified, and the polynucleotide chain therefore sequenced, during this translocation. Difficulties to be overcome include slowing down the movement of the chain through the pore so that the electronic detector can record the individual bases moving past. In addition, computer algorithms that can process the volume of data resulting from scanning up to 70 000 nucleotides in a matter of minutes need to be developed. S de B (http://www.economist.com/science/tq/displayStory.cfm?Story_ID=662230&CFID=1206325&CFTOKEN=54743604; http://golgi.harvard.edu/branton/nanopore.html)" @default.
- W1993575755 created "2016-06-24" @default.
- W1993575755 creator A5001548815 @default.
- W1993575755 date "2001-09-01" @default.
- W1993575755 modified "2023-09-24" @default.
- W1993575755 title "One hour, one genome" @default.
- W1993575755 doi "https://doi.org/10.1016/s0968-0004(01)01967-3" @default.
- W1993575755 hasPublicationYear "2001" @default.
- W1993575755 type Work @default.
- W1993575755 sameAs 1993575755 @default.
- W1993575755 citedByCount "0" @default.
- W1993575755 crossrefType "journal-article" @default.
- W1993575755 hasAuthorship W1993575755A5001548815 @default.
- W1993575755 hasConcept C104317684 @default.
- W1993575755 hasConcept C12554922 @default.
- W1993575755 hasConcept C126513998 @default.
- W1993575755 hasConcept C127413603 @default.
- W1993575755 hasConcept C141231307 @default.
- W1993575755 hasConcept C141795571 @default.
- W1993575755 hasConcept C171250308 @default.
- W1993575755 hasConcept C185592680 @default.
- W1993575755 hasConcept C188198153 @default.
- W1993575755 hasConcept C192157962 @default.
- W1993575755 hasConcept C192562407 @default.
- W1993575755 hasConcept C39944091 @default.
- W1993575755 hasConcept C41625074 @default.
- W1993575755 hasConcept C512185932 @default.
- W1993575755 hasConcept C51679486 @default.
- W1993575755 hasConcept C552990157 @default.
- W1993575755 hasConcept C55493867 @default.
- W1993575755 hasConcept C70721500 @default.
- W1993575755 hasConcept C78519656 @default.
- W1993575755 hasConcept C86803240 @default.
- W1993575755 hasConceptScore W1993575755C104317684 @default.
- W1993575755 hasConceptScore W1993575755C12554922 @default.
- W1993575755 hasConceptScore W1993575755C126513998 @default.
- W1993575755 hasConceptScore W1993575755C127413603 @default.
- W1993575755 hasConceptScore W1993575755C141231307 @default.
- W1993575755 hasConceptScore W1993575755C141795571 @default.
- W1993575755 hasConceptScore W1993575755C171250308 @default.
- W1993575755 hasConceptScore W1993575755C185592680 @default.
- W1993575755 hasConceptScore W1993575755C188198153 @default.
- W1993575755 hasConceptScore W1993575755C192157962 @default.
- W1993575755 hasConceptScore W1993575755C192562407 @default.
- W1993575755 hasConceptScore W1993575755C39944091 @default.
- W1993575755 hasConceptScore W1993575755C41625074 @default.
- W1993575755 hasConceptScore W1993575755C512185932 @default.
- W1993575755 hasConceptScore W1993575755C51679486 @default.
- W1993575755 hasConceptScore W1993575755C552990157 @default.
- W1993575755 hasConceptScore W1993575755C55493867 @default.
- W1993575755 hasConceptScore W1993575755C70721500 @default.
- W1993575755 hasConceptScore W1993575755C78519656 @default.
- W1993575755 hasConceptScore W1993575755C86803240 @default.
- W1993575755 hasIssue "9" @default.
- W1993575755 hasLocation W19935757551 @default.
- W1993575755 hasOpenAccess W1993575755 @default.
- W1993575755 hasPrimaryLocation W19935757551 @default.
- W1993575755 hasRelatedWork W2032711662 @default.
- W1993575755 hasRelatedWork W2086290527 @default.
- W1993575755 hasRelatedWork W2495711731 @default.
- W1993575755 hasRelatedWork W2559981067 @default.
- W1993575755 hasRelatedWork W2942826041 @default.
- W1993575755 hasRelatedWork W2964187307 @default.
- W1993575755 hasRelatedWork W3087086484 @default.
- W1993575755 hasRelatedWork W3146423745 @default.
- W1993575755 hasRelatedWork W3149795039 @default.
- W1993575755 hasRelatedWork W4320710005 @default.
- W1993575755 hasVolume "26" @default.
- W1993575755 isParatext "false" @default.
- W1993575755 isRetracted "false" @default.
- W1993575755 magId "1993575755" @default.
- W1993575755 workType "article" @default.