Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993582395> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W1993582395 endingPage "339" @default.
- W1993582395 startingPage "331" @default.
- W1993582395 abstract "The intact epicuticles of Strongyloides ratti stage-3 larvae and Trichinella spiralis stage-1 larvae were found to have a surface net negative charge. Ultrastructural studies on S. ratti using cationized ferritin and ruthenium red showed the negative charge to be dense and uniformly distributed over the epicuticular surface. Staining with acetic acid-ferric oxide hydrosol occurred at pH 1.65 and suggests that amino acid carboxyl groups were not responsible for the negative charge property. Alcian blue staining occurred at pH 0.5 and at a critical electrolyte concentration (CEC) of 0.9 M MgCl2, a property similar to that of highly sulfated mucopolysaccharides such as the proteoglycan keratan sulfate. In contrast, T. spiralis larvae failed to stain with alcian blue below pH 5.0 or at a CEC of 0.1 M, suggesting its negative charge is associated with dissociated amino acid carboxyl groups. Attempts to remove the negative charge-bearing components in the epicuticle of S. ratti by detergents, organic solvents, denaturing agents, proteases, uronidases, neuraminidases, and lipases were unsuccessful. The presence of elastin in the S. ratti larval outer cortical layer was indicated by its vulnerability to elastase and its reaction to aldehyde fuchsin-alcian blue stain. These results show that the epicuticle of S. ratti is not a typical cell membrane, although it appears to have ultrastructural similarities. It is suggested that the association of highly sulfated mucopolysaccharides with the epicuticular surface of free-living nematodes such as S. ratti L3 may reflect a greater need to protect against surface desiccation. It is also postulated that the highly negatively charged surface may have anticomplementary and anticoagulation effects." @default.
- W1993582395 created "2016-06-24" @default.
- W1993582395 creator A5012022615 @default.
- W1993582395 creator A5033150479 @default.
- W1993582395 creator A5060907589 @default.
- W1993582395 date "1983-06-01" @default.
- W1993582395 modified "2023-10-01" @default.
- W1993582395 title "Strongyloides ratti and Trichinella spiralis: Net charge of epicuticle" @default.
- W1993582395 cites W1971233190 @default.
- W1993582395 cites W1988952913 @default.
- W1993582395 cites W1996975390 @default.
- W1993582395 cites W2021975128 @default.
- W1993582395 cites W2025318146 @default.
- W1993582395 cites W2035467252 @default.
- W1993582395 cites W2087580666 @default.
- W1993582395 cites W2119294399 @default.
- W1993582395 cites W2160993941 @default.
- W1993582395 cites W2161395512 @default.
- W1993582395 cites W2326710875 @default.
- W1993582395 cites W2333119721 @default.
- W1993582395 cites W2334072287 @default.
- W1993582395 doi "https://doi.org/10.1016/0014-4894(83)90030-9" @default.
- W1993582395 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6221941" @default.
- W1993582395 hasPublicationYear "1983" @default.
- W1993582395 type Work @default.
- W1993582395 sameAs 1993582395 @default.
- W1993582395 citedByCount "40" @default.
- W1993582395 countsByYear W19935823952017 @default.
- W1993582395 countsByYear W19935823952020 @default.
- W1993582395 countsByYear W19935823952023 @default.
- W1993582395 crossrefType "journal-article" @default.
- W1993582395 hasAuthorship W1993582395A5012022615 @default.
- W1993582395 hasAuthorship W1993582395A5033150479 @default.
- W1993582395 hasAuthorship W1993582395A5060907589 @default.
- W1993582395 hasConcept C173758957 @default.
- W1993582395 hasConcept C178790620 @default.
- W1993582395 hasConcept C185592680 @default.
- W1993582395 hasConcept C2779657890 @default.
- W1993582395 hasConcept C2781320125 @default.
- W1993582395 hasConcept C519063684 @default.
- W1993582395 hasConcept C54355233 @default.
- W1993582395 hasConcept C55493867 @default.
- W1993582395 hasConcept C59822182 @default.
- W1993582395 hasConcept C74864618 @default.
- W1993582395 hasConcept C86803240 @default.
- W1993582395 hasConcept C87555872 @default.
- W1993582395 hasConcept C89423630 @default.
- W1993582395 hasConceptScore W1993582395C173758957 @default.
- W1993582395 hasConceptScore W1993582395C178790620 @default.
- W1993582395 hasConceptScore W1993582395C185592680 @default.
- W1993582395 hasConceptScore W1993582395C2779657890 @default.
- W1993582395 hasConceptScore W1993582395C2781320125 @default.
- W1993582395 hasConceptScore W1993582395C519063684 @default.
- W1993582395 hasConceptScore W1993582395C54355233 @default.
- W1993582395 hasConceptScore W1993582395C55493867 @default.
- W1993582395 hasConceptScore W1993582395C59822182 @default.
- W1993582395 hasConceptScore W1993582395C74864618 @default.
- W1993582395 hasConceptScore W1993582395C86803240 @default.
- W1993582395 hasConceptScore W1993582395C87555872 @default.
- W1993582395 hasConceptScore W1993582395C89423630 @default.
- W1993582395 hasIssue "3" @default.
- W1993582395 hasLocation W19935823951 @default.
- W1993582395 hasLocation W19935823952 @default.
- W1993582395 hasOpenAccess W1993582395 @default.
- W1993582395 hasPrimaryLocation W19935823951 @default.
- W1993582395 hasRelatedWork W145185060 @default.
- W1993582395 hasRelatedWork W1520249766 @default.
- W1993582395 hasRelatedWork W1538132158 @default.
- W1993582395 hasRelatedWork W1970919307 @default.
- W1993582395 hasRelatedWork W1996641622 @default.
- W1993582395 hasRelatedWork W2091749576 @default.
- W1993582395 hasRelatedWork W2325742463 @default.
- W1993582395 hasRelatedWork W2326020785 @default.
- W1993582395 hasRelatedWork W2412484576 @default.
- W1993582395 hasRelatedWork W2431866875 @default.
- W1993582395 hasVolume "55" @default.
- W1993582395 isParatext "false" @default.
- W1993582395 isRetracted "false" @default.
- W1993582395 magId "1993582395" @default.
- W1993582395 workType "article" @default.