FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993661906> ?p ?o ?g. }

• W1993661906 endingPage "5812" @default.
• W1993661906 startingPage "5800" @default.
• W1993661906 abstract "In this paper, a simple and innovative electrochemical hydrogen peroxide biosensor has been proposed using catalase (CATpp) derived from Pichia pastoris as bioelectrocatalyst. The model biocomponent was immobilized on gold nanoparticle nanotubes (AuNPNTs) and polythiophene composite using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide and N-hydroxysuccinimide (EDC-NHS) coupling reagent. In this present work, we have successfully synthesized gold nanoparticles (AuNPs) by ultrasonic irradiation. The tubular gold nanostructures containing coalesced AuNPs were obtained by sacrificial template synthesis. The assembly of AuNPNTs onto the graphite (Gr) electrode was achieved via S-Au chemisorption. The latter was pre-coated with electropolymerized thiophene (PTh) to enable S groups to bind AuNPNTs. The combination of AuNPNTs-PTh, i.e., an inorganic-organic hybrid, provides a stable enzyme immobilization platform. The physical morphology of the fabricated biosensor Gr/PTh/AuNPNTs/EDC-NHS/CATpp was investigated using scanning electron microscopy and energy-dispersive microscopy. The analytical performance of the bioelectrode was examined using cyclic voltammetry, differential pulse voltammetry and chronoamperometry. Operational parameters such as working potential, pH, and thermal stability of the modified electrode were examined. The beneficial analytical characteristics of the proposed electrode were demonstrated. Our results indicate that the Gr/PTh/AuNPNTs/EDC-NHS/CATpp bioelectrode exhibits a wide linear range from 0.05 mM to 18.5 mM of H2O2, fast response time of 7 s, excellent sensitivity of 26.2 mA mM(-1) cm(-2), good detection limit of 0.12 μM and good Michaelis-Menten constant of 1.4 mM. In addition, the bioelectrode displayed good repeatability, high stability and acceptable reproducibility, which can be attributed to the AuNPNTs-PTh composite that provides a biocompatible micro-environment." @default.
• W1993661906 created "2016-06-24" @default.
• W1993661906 creator A5007935648 @default.
• W1993661906 creator A5008558712 @default.
• W1993661906 creator A5027456797 @default.
• W1993661906 creator A5054870663 @default.
• W1993661906 creator A5057200254 @default.
• W1993661906 creator A5068652338 @default.
• W1993661906 creator A5085740893 @default.
• W1993661906 date "2014-01-01" @default.
• W1993661906 modified "2023-09-27" @default.
• W1993661906 title "Development of a simple bioelectrode for the electrochemical detection of hydrogen peroxide using Pichia pastoris catalase immobilized on gold nanoparticle nanotubes and polythiophene hybrid" @default.
• W1993661906 cites W1741319179 @default.
• W1993661906 cites W191793640 @default.
• W1993661906 cites W1966177413 @default.
• W1993661906 cites W1970869721 @default.
• W1993661906 cites W1975943401 @default.
• W1993661906 cites W1979763515 @default.
• W1993661906 cites W1980181517 @default.
• W1993661906 cites W1980359364 @default.
• W1993661906 cites W1989758864 @default.
• W1993661906 cites W1989890220 @default.
• W1993661906 cites W1994115200 @default.
• W1993661906 cites W2001673909 @default.
• W1993661906 cites W2003047846 @default.
• W1993661906 cites W2006531691 @default.
• W1993661906 cites W2008467847 @default.
• W1993661906 cites W2018614111 @default.
• W1993661906 cites W2018858346 @default.
• W1993661906 cites W2022446334 @default.
• W1993661906 cites W2026594734 @default.
• W1993661906 cites W2027325112 @default.
• W1993661906 cites W2032697550 @default.
• W1993661906 cites W2039699713 @default.
• W1993661906 cites W2042104005 @default.
• W1993661906 cites W2048985834 @default.
• W1993661906 cites W2055010149 @default.
• W1993661906 cites W2059140613 @default.
• W1993661906 cites W2088233731 @default.
• W1993661906 cites W2090006558 @default.
• W1993661906 cites W2092654431 @default.
• W1993661906 cites W2098223768 @default.
• W1993661906 cites W2102906117 @default.
• W1993661906 cites W2103348339 @default.
• W1993661906 cites W2110165736 @default.
• W1993661906 cites W2135724420 @default.
• W1993661906 cites W2167206974 @default.
• W1993661906 cites W2171696128 @default.
• W1993661906 cites W2316681226 @default.
• W1993661906 cites W3021566866 @default.
• W1993661906 cites W4241803171 @default.
• W1993661906 cites W4242654813 @default.
• W1993661906 cites W4382023718 @default.
• W1993661906 cites W4382397092 @default.
• W1993661906 doi "https://doi.org/10.1039/c4an01262c" @default.
• W1993661906 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25208248" @default.
• W1993661906 hasPublicationYear "2014" @default.
• W1993661906 type Work @default.
• W1993661906 sameAs 1993661906 @default.
• W1993661906 citedByCount "29" @default.
• W1993661906 countsByYear W19936619062015 @default.
• W1993661906 countsByYear W19936619062016 @default.
• W1993661906 countsByYear W19936619062017 @default.
• W1993661906 countsByYear W19936619062018 @default.
• W1993661906 countsByYear W19936619062019 @default.
• W1993661906 countsByYear W19936619062020 @default.
• W1993661906 countsByYear W19936619062023 @default.
• W1993661906 crossrefType "journal-article" @default.
• W1993661906 hasAuthorship W1993661906A5007935648 @default.
• W1993661906 hasAuthorship W1993661906A5008558712 @default.
• W1993661906 hasAuthorship W1993661906A5027456797 @default.
• W1993661906 hasAuthorship W1993661906A5054870663 @default.
• W1993661906 hasAuthorship W1993661906A5057200254 @default.
• W1993661906 hasAuthorship W1993661906A5068652338 @default.
• W1993661906 hasAuthorship W1993661906A5085740893 @default.
• W1993661906 hasConcept C104819515 @default.
• W1993661906 hasConcept C113196181 @default.
• W1993661906 hasConcept C119128265 @default.
• W1993661906 hasConcept C133495861 @default.
• W1993661906 hasConcept C13965031 @default.
• W1993661906 hasConcept C147789679 @default.
• W1993661906 hasConcept C155672457 @default.
• W1993661906 hasConcept C160756335 @default.
• W1993661906 hasConcept C171250308 @default.
• W1993661906 hasConcept C17525397 @default.
• W1993661906 hasConcept C178790620 @default.
• W1993661906 hasConcept C185592680 @default.
• W1993661906 hasConcept C192562407 @default.
• W1993661906 hasConcept C2777869211 @default.
• W1993661906 hasConcept C2778141235 @default.
• W1993661906 hasConcept C2778402822 @default.
• W1993661906 hasConcept C2779262620 @default.
• W1993661906 hasConcept C2779525745 @default.
• W1993661906 hasConcept C43617362 @default.
• W1993661906 hasConcept C52859227 @default.
• W1993661906 hasConcept C533411734 @default.
• W1993661906 hasConceptScore W1993661906C104819515 @default.
• W1993661906 hasConceptScore W1993661906C113196181 @default.

• next