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• W1993665928 abstract "Tertiary structure of the recombinant glutamate dehydrogenase from Thermococcus kodakaraensis KOD1 (Tk-rGDH) converts into an intact form induced by the heat treatment. This phenomenon, heat-induced structural maturation, means that high temperature plays an important role in the proper folding and oligomerization of Tk-rGDH. In this work, we analyzed the heat-induced structural maturation of Tk-rGDH by differential scanning microcalorimetry (DSC), circular dichroism (CD), and activity measurements. In DSC measurements, the peak of adsorption of non-heated Tk-rGDH (nh-Tk-rGDH) was two times smaller than that of Tk-rGDH heated at 70°C for 30 min (h-Tk-rGDH). The transition temperature (Tm) of h-Tk-rGDH was 115°C, which was about 3°C higher than that of nh-Tk-rGDH. In the presence of 0.5 M NaCl, the nh-Tk-rGDH showed two peaks at 107°C and 114°C, while the h-Tk-rGDH showed a single peak at 115.7°C. The heat-induced conformational change process was monitored by changes in CD intensity at 222 nm, and the result showed that heat-induced structural maturation is irreversible. The heat treatment at 70°C showed the highest enhancement in activity, which was 15% larger than that of heat-treated Tk-rGDH at 40°C. The results indicate that heat-induced structural maturation involves an irreversible process, transforming the non-heated form to the stable and active form." @default.
• W1993665928 created "2016-06-24" @default.
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• W1993665928 date "2004-01-01" @default.
• W1993665928 modified "2023-10-06" @default.
• W1993665928 title "Biophysical analysis of heat-induced structural maturation of glutamate dehydrogenase from a hyperthermophilic archaeon" @default.
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• W1993665928 doi "https://doi.org/10.1016/s1389-1723(04)70210-8" @default.
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