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- W1993669854 abstract "We have constructed a yeast artificial chromosome (YAC) library derived from hull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8 × 12 positions) and in 2 × 224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones. The proportion of chimeric YACs was estimated from the analysis of a subset of the positive clones: 3 of 9 YACs were found to contain noncontiguous DNA." @default.
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- W1993669854 date "1993-11-01" @default.
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- W1993669854 title "Construction of a Bovine Genomic Library of Large Yeast Artificial Chromosome Clones" @default.
- W1993669854 doi "https://doi.org/10.1006/geno.1993.1465" @default.
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