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• W1993671811 abstract "A soluble extract of adult chick sciatic nerve was added to cultures of dissociated embryonic chick muscle cells in order to determine possible neurotrophic influences of the extract on the differentiation and maintenance of cell structure, synthesis of macromolecules, and acetylcholinesterase activity of the muscle cells. The rate and degree of morphological differentiation and maturation, synthesis of dexoyribonucleic acid and protein, and acetylcholinesterase activity were all enhanced by the extract. Also, fully matured muscle fibers survived for longer periods of time in cultures with the nerve extract than in control cultures. Furthermore, muscle degeneration was reversed by addition of the extract to the culture medium of degenerating muscles. The chemical nature and molecular size of the neurotrophic principle in the extract was further analyzed by enzymatic digestion or Diaflo membrane filtration of the extract. The active substance appears to be a molecular weight between 10,000 and 50,000 and is probably glycoprotein. It is concluded that a neurotrophic factor capable of substituting for innervation can be extracted from nerve tissue." @default.
• W1993671811 created "2016-06-24" @default.
• W1993671811 creator A5087761262 @default.
• W1993671811 date "1976-02-01" @default.
• W1993671811 modified "2023-09-23" @default.
• W1993671811 title "Neurotrophic effects of sciatic nerve extracts on muscle development in culture" @default.
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• W1993671811 doi "https://doi.org/10.1016/0014-4886(76)90012-1" @default.
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