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- W1993682762 abstract "We describe the first fluorescence lifetime images of cells. To demonstrate this new capability we measured intracellular images of Ca2+ in COS cells based on the Ca2+-dependent fluorescence lifetime of Quin-2. Apparent fluorescence lifetimes were measured by the phase-modulation method using a gain-modulated image intensifier and a slow-scan CCD camera. We describe methods to correct the images for photobleaching during acquisition of the data, and to correct for the position-dependent response of the image intensifier. The phase angle Quin-2 images were found to yield lower than expected Ca2+ concentrations, which appears to be the result of the formation of fluorescent photoproducts by Quin-2. Fluorescence lifetime imaging (FLIM) does not require wavelength-ratiometric probes and appears to provide new opportunities for chemical imaging of cells." @default.
- W1993682762 created "2016-06-24" @default.
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- W1993682762 date "1994-01-01" @default.
- W1993682762 modified "2023-10-16" @default.
- W1993682762 title "Fluorescence lifetime imaging of intracellular calcium in COS cells using Quin-2" @default.
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- W1993682762 doi "https://doi.org/10.1016/0143-4160(94)90100-7" @default.
- W1993682762 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6906927" @default.
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