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• W1993691900 abstract "Article15 May 2000free access HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation Wengong Wang Wengong Wang Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA Search for more papers by this author M.Craig Caldwell M.Craig Caldwell Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA Search for more papers by this author Shankung Lin Shankung Lin Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA Search for more papers by this author Henry Furneaux Henry Furneaux Program in Molecular Pharmacology and Therapeutics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10021 USA Search for more papers by this author Myriam Gorospe Corresponding Author Myriam Gorospe Box 12, LBC, NIA, NIH, 5600 Nathan Shock Drive, Baltimore, MD, 21224 USA Search for more papers by this author Wengong Wang Wengong Wang Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA Search for more papers by this author M.Craig Caldwell M.Craig Caldwell Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA Search for more papers by this author Shankung Lin Shankung Lin Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA Search for more papers by this author Henry Furneaux Henry Furneaux Program in Molecular Pharmacology and Therapeutics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10021 USA Search for more papers by this author Myriam Gorospe Corresponding Author Myriam Gorospe Box 12, LBC, NIA, NIH, 5600 Nathan Shock Drive, Baltimore, MD, 21224 USA Search for more papers by this author Author Information Wengong Wang1, M.Craig Caldwell1, Shankung Lin1, Henry Furneaux2 and Myriam Gorospe 3 1Laboratory of Biological Chemistry, NIA, NIH, Baltimore, MD, 21224 USA 2Program in Molecular Pharmacology and Therapeutics, Memorial Sloan-Kettering Cancer Center, New York, NY, 10021 USA 3Box 12, LBC, NIA, NIH, 5600 Nathan Shock Drive, Baltimore, MD, 21224 USA *Corresponding author. E-mail: [email protected] The EMBO Journal (2000)19:2340-2350https://doi.org/10.1093/emboj/19.10.2340 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Colorectal carcinoma RKO cells expressing reduced levels of the RNA-binding protein HuR (ASHuR) displayed markedly reduced growth. In synchronous RKO populations, HuR was almost exclusively nuclear during early G1, increasing in the cytoplasm during late G1, S and G2. The expression and half-life of mRNAs encoding cyclins A and B1 similarly increased during S and G2, then declined, indicating that mRNA stabilization contributed to their cell cycle-regulated expression. In gel-shift assays using radiolabeled cyclin RNA transcripts and RKO protein extracts, only those transcripts corresponding to the 3′-untranslated regions of cyclins A and B1 formed RNA–protein complexes in a cell cycle-dependent fashion. HuR directly bound mRNAs encoding cyclins A and B1, as anti-HuR antibodies supershifted such RNA–protein complexes. Importantly, the expression and half-life of mRNAs encoding cyclins A and B1 were reduced in ASHuR RKO cells. Our results indicate that HuR may play a critical role in cell proliferation, at least in part by mediating cell cycle-dependent stabilization of mRNAs encoding cyclins A and B1. Introduction The mamalian cell division cycle is governed through the orchestrated activation and inactivation of cyclin-dependent kinases (cdks), whose activity is modulated by a host of regulatory events (Johnson and Walker, 1999). Negative regulators of cdk include cdk-inhibitory proteins, and positive regulators include cyclins, their catalytic partners. During the G1 phase, cyclins D1, D2 and D3 form complexes with cdk4 or cdk6, and cyclin E with cdk2; these complexes function as Rb kinases, modulating the activity of E2F and hence the expression of proliferative genes. Cyclin A associates with cdk2 during the S phase, and with cdc2 (cdk1) at the S–G2 boundary and into G2. Progression through G2, culminating in mitosis, further requires that cdc2 form complexes with cyclins B1 and B2. Given the central role of cyclins in controlling cell cycle progression, their expression during the cell division cycle is tightly regulated. The transcriptional regulation of cyclins D1 and E, which are induced after mitogenic stimulation, has been extensively documented (Ohtani et al., 1995; Guttridge et al., 1999). Likewise, some studies have reported cell cycle-dependent transcriptional regulation of cyclin A (Henglein et al., 1994; Zwicker et al., 1995) and of cyclin B1 (Hwang et al., 1995, 1998; Piaggio et al., 1995). Post-transcriptional events controlling cyclin expression include their rapid and specific proteolysis by the ubiquitin–proteasome pathway (King et al., 1996; Koepp et al., 1999). However, it has long been recognized that the expression of cyclins and cell cycle-related proteins is also critically regulated by altering the stability of their mRNAs. Such regulatory mechanisms have been proposed for cyclin D1 (Lebwohl et al., 1994; Hashemolhosseini et al., 1998; Hosokawa et al., 1998), cyclin E (Oda et al., 1995), cyclin A (Howe et al., 1995; Maity et al., 1997), cyclin B1 (Trembley et al., 1994; Maity et al., 1995), p21 (Schwaller et al., 1995; Esposito et al., 1997; Gorospe et al., 1998), p27 (Baghdassarian et al., 1999), cdk2 (Oda et al., 1995), cdc2 and cdc25 (Datta et al., 1992), among other cell cycle-regulatory genes. Indeed, the stability of some cyclin mRNAs varies as a function of the cell cycle (Maity et al., 1995, 1997). The process of mRNA turnover is increasingly recognized as critical for regulating the expression of many genes during processes such as immune-cell activation, response to mitogens, differentiation, and cell cycle regulation (Malter and Hong, 1991; Schiavi et al., 1992; Ross, 1995; Liebhaber, 1997). Many mRNAs subject to message turnover identified to date bear AU-rich elements (AREs) in their 3′-untranslated regions (3′ UTRs), which often also contain the pentamer AUUUA (Caput et al., 1986; Shaw and Kamen, 1986; Shyu et al., 1989; Lagnado et al., 1994; Chen and Shyu, 1995; Xu et al., 1997). These sequences have been shown to participate in rapid changes in the steady-state levels of mRNAs encoding critical growth-response genes (Treisman et al., 1985), cytokines (Wodnar-Filipowicz and Moroni, 1990; Henics et al., 1994) and cell-cycle regulatory proteins (Datta et al., 1992; Lebwohl et al., 1994; Markiewicz et al., 1994; Nemer and Stuebing, 1996). Among the best characterized ARE-binding proteins are the Elav/Hu family of RNA-binding proteins, composed of the ubiquitously expressed HuR (HuA) and the neuronal specific Hel-N1 (HuB), HuC and HuD (Levine et al., 1993; Good, 1995; Chung et al., 1996; Ma et al., 1996; Antic and Keene, 1997). Hu proteins were first identified as specific tumor antigens in cancers (particularly lung carcinomas) of individuals with paraneoplastic neurological disorder (Dalmau et al., 1990; Szabo et al., 1991). Patients developed autoantibodies against Hu proteins, which penetrated the blood–brain barrier and led to neuronal degeneration. The features of paraneoplastic disease suggest that Hu proteins may play a pivotal role in controlling the expression of growth-regulatory genes. Indeed, Hu proteins have been found to bind to critical ARE-containing mRNAs and either stabilize them, enhance their translation, or both (Fan and Steitz, 1998a; Peng et al., 1998; Ford et al., 1999). For example, there is evidence for binding, stabilization and/or enhanced translation of GLUT-1, neurofilament M and c-myc mRNAs by Hel-N1 (Levine et al., 1993; Jain et al., 1997; Antic et al., 1999); N-myc, GAP-43 and tau mRNAs by HuD (Chung et al., 1997; Aranda-Abreu et al., 1999; Lazarova et al., 1999); and c-fos, PAI-2, VEGF, p21 and c-myc mRNAs by HuR (Joseph et al., 1998; Levy et al., 1998; Peng et al., 1998; Maurer et al., 1999; Wang et al., 2000). In addition, the intracellular distribution of Elav proteins varies (Antic and Keene, 1997; Keene, 1999). HuR, which bears the recently described nuclear shuttling sequence HNS (Fan and Steitz, 1998b), is primarily nuclear, but can redistribute to the cytoplasm (Atasoy et al., 1998; Fan and Steitz, 1998b; Peng et al., 1998). While it is becoming apparent that the neuronal members (Hel-N1, HuC and HuD) function in the terminal differentiation of neurons (Aranda-Abreu et al., 1999), the role of HuR in cell proliferation or differentiation has not been directly addressed. We recently demonstrated that HuR‘s cytoplasmic localization increased under conditions of stress and further showed that this redistribution was coupled with its ability to bind to and stabilize the mRNA encoding the cdk inhibitor p21 (Wang et al., 2000). During the course of those studies, we generated cells whose HuR levels were downregulated through expression of an antisense (AS) HuR transcript and observed that ASHuR cells exhibited markedly reduced proliferation rates. In the present study we have directly examined the influence of HuR on cell proliferation. Our observations reveal the cell cycle dependency of (i) the levels of cytoplasmic HuR, (ii) the formation of RNA–protein complexes with 3′ UTR transcripts of cyclins A and B1 and (iii) the stabilization of mRNAs encoding cyclins A and B1. Importantly, ASHuR-expressing cells exhibited decreased binding to their respective mRNAs, significantly reduced half-lives for mRNAs encoding cyclins A and B1, lower levels of cyclins A and B1, and, consequently, reduced proliferation rates. Our observations strongly support HuR’s function in controlling the expression of cyclins A and cyclin B1 through upregulation of the half-life of their mRNAs. Thus, our observations not only illustrate the importance of HuR in cell proliferation, but also yield novel insight into the post-transcriptional regulation of cyclin A and cyclin B1 expression. Results RKO cells expressing reduced HuR levels exhibit slower cell proliferation Several RKO clonal lines expressing lower levels of HuR by transfection with an antisense transcript complementary to HuR mRNA were generated in our laboratory during the course of earlier studies (Wang et al., 2000). HuR expression in two such ASHuR clones, AS.17 and AS.11 (Figure 1A), was 75% (3-fold) and 80% (4-fold) lower than that seen in the control zeo population. This reduction was specific for HuR as the expression of AUF1, another RNA-binding protein (Zhang et al., 1993), remained unchanged (Figure 1A). AS.17 and AS.11, as well as other ASHuR clonal isolates, exhibited markedly decreased proliferation rates and displayed greater S and G2/M compartments (Figure 1B and C). The differences in population growth were unlikely to result from enhanced death of ASHuR cells, since Trypan blue-exclusion assays revealed <2% non-viable cells in all lines. Instead, we hypothesized that HuR may affect the expression of genes involved in cell cycle progression and sought to investigate this function of HuR further. Figure 1.RKO cells expressing reduced HuR show reduced growth rates. (A) Western blot analysis depicting HuR and AUF1 expression in RKO cells transfected with the empty pSVzeo vector (zeo), and in two RKO clonal lines transfected with pSVzeo(–) HuR to express an antisense HuR transcript (AS.17 and AS.11). Blots were sequentially stripped and rehybridized to assess the expression of AUF1 and actin. (B) Proliferation rates in RKO zeo, AS.17 and AS.11 cells, measured after seeding 10 000 cells and counting at 24 h intervals using a hemacytometer. (C) Representative FACS histograms of RKO zeo, AS.17 and AS.11 cultures undergoing logarithmic growth. Data represent the mean ± SEM of four independent experiments. Download figure Download PowerPoint Cell cycle-dependent cytoplasmic localization of HuR To explore the potential role of HuR in cell proliferation, we first examined the expression of HuR throughout the cell division cycle. To this end, RKO cells were subjected to a synchronization regimen consisting of 3-day culture in serum-free medium that markedly enriched the G1-cell compartment, from 30–35% in asynchronous cultures to >75% after serum starvation, as described previously (Gorospe and Holbrook, 1996). G1-enriched, growth-inhibited cultures were released from arrest by the addition of serum (final 10%) and cell cycle progression was monitored by FACS analysis (described in Gorospe and Holbrook, 1996) and by measuring incorporation of [3H]thymidine (Figure 2A). As observed, the highest [3H]thymidine incorporation was seen between 12 and 24 h. It was estimated that, under these conditions, RKO cells required ∼28 h to complete the entire first division cycle; synchrony was rapidly lost in subsequent rounds of division. Figure 2.Cell cycle-dependent cytoplasmic localization of HuR. (A) Thymidine incorporation in synchronous RKO zeo populations after serum addition. Data represent the mean ± SEM of four independent experiments. (B) Western blot analysis of HuR (34 kDa) levels in the cytoplasmic (Cyto.) and nuclear (Nuc.) fractions of RKO cells that were either serum starved for 3 days, then serum stimulated for the times indicated, or asynchronously growing (Asyn.). To monitor the quality of the fractionation procedure and the evenness in loading and transfer among samples, membranes were stripped and rehybridized to detect either actin (cytoplasmic, 43 kDa) or BAF57c (nuclear, 57 kDa). (C) Western blot analysis of HuR levels in the cytoplasmic and nuclear fractions of synchronous mouse embryo fibroblast populations that were either serum starved for 3 days, then stimulated by addition of 10% serum, or grown to high density (high), then replated at low density. Download figure Download PowerPoint By Western blot analysis, whole-cell protein lysates revealed no substantial cell cycle-dependent differences in HuR expression (not shown). However, several recent reports have provided evidence that HuR is transported between the nucleus and the cytoplasm (Atasoy et al., 1998; Fan and Steitz, 1998b; Peng et al., 1998; Keene, 1999; Wang et al., 2000). As these translocation events may be linked to HuR‘s function, we directly examined the subcellular localization of HuR as a function of the cell cycle stage. Interestingly, HuR was almost entirely nuclear during the first hours after serum addition (early G1), but by 6–12 h, when cells begin to replicate their DNA and progress through S phase, HuR’s presence in the cytoplasm increased dramatically. A concomitant reduction, though moderate, was observed in nuclear HuR (Figure 2B). Approximately at the time when cells are completing G2, HuR's presence in the cytoplasm decreased (24 h time point), becoming lower again by the following G1 phase (32 h). Subsequent divisions occurred with less synchrony and cultures rapidly approached confluence. Serum addition profoundly alters many cellular processes. To rule out the possibility that HuR‘s translocation was merely due to such ’serum effects‘ and was due, instead, to the cells’ progression through their division cycle, we monitored the distribution of HuR in cultures that were synchronized by alternative means. Thus, we employed mouse embryo fibroblasts that could be synchronized either by growth to high density in medium containing 10% serum (91% G1 cells by 48 h after reaching confluence), a synchronization regimen to which RKO cells are not amenable, or serum deprivation for 3 days (87% G1 cells). Release by addition of serum or replating at low density, respectively, led to cell cycle progression, as monitored by FACS analysis (not shown), with one division cycle requiring ∼36 h. Despite differences in cell cycle duration, HuR essentially displayed the same subcellular distribution: its abundance in the cytoplasm was low during early and mid G1, but subsequently increased, reaching a maximum level during the time period corresponding to the S and G2 phases; it then decreased again at the onset of the following G1 (Figure 2C). In conclusion, HuR's presence in the cytoplasm varied in a cell cycle-dependent manner and was highest during the S and G2 phases. Cyclin mRNA expression and stability during cell cycle progression We next examined the expression of cyclins in RKO cells following release from serum starvation. Shown in Figure 3A are Northern blot analyses of the expression and relative induction of mRNAs encoding cyclins D1, E, A and B1. Throughout the time period studied, only a moderate fluctuation in the expression of mRNAs encoding cyclins D1 and E was observed, with maximal inductions of 4- and 2-fold, respectively, over the levels seen at time 0. Expression of cyclins D2 and D3 was unchanged (not shown). In contrast, mRNAs encoding cyclins A and B1 were greatly induced following serum addition: expression of each transcript between 10 and 20 h was 14- to 16-fold higher than that seen before serum induction. Figure 3.Cyclin mRNA expression and stability during the cell division cycle. (A) Left: representative Northern blot analysis of the expression of cyclins D1, E, A and B1. After a 3 day synchronization period, cells were serum stimulated for the times shown, whereupon RNA was extracted for analysis. Blots were sequentially stripped and rehybridized to detect each of the transcripts shown; 18S rRNA signals served to quantitate differences in loading and transfer among samples. Right: quantitation of the levels of each cyclin mRNA from three independent experiments. Data are represented as fold induction in mRNA levels relative to those at time 0 (before serum addition). (B) The half-lives of cyclin A and cyclin B1 mRNAs in either asynchronous (asyn.) or synchronous populations (serum stimulated for the times indicated) were calculated after adding 2 μg/ml actinomycin D after each serum stimulation period, preparing RNA at the times indicated thereafter, measuring the remaining signals of cyclin A and cyclin B1 mRNAs by Northern blot analysis, normalizing them to 18S rRNA, and plotting them on a logarithmic scale (inset). mRNA half-lives at each time following serum stimulation are indicated in parentheses. Download figure Download PowerPoint Such increases in the steady-state levels of mRNAs encoding cylins A and B1 were due, at least in part, to changes in the turnover rates of each transcript. When the half-life of each transcript was calculated (by standard actinomycin D-chase analysis) at different times after serum addition, they were found to vary significantly (Figure 3B). Both for cyclin A and cyclin B1 mRNAs, half-lives were lowest (2.2 and 2.8 h, respectively) before serum addition (0 h), when most cells were in early G1. After serum addition half-lives progressively increased, with the longest half-lives of >12 h seen by 18 h (S-phase peak), and decreased afterwards, as cells exited G2 (Figure 3B). HuR complexes with cyclin A and cyclin B1 transcripts in vitro and in vivo. Cell cycle-dependent binding to cyclin A and cyclin B1 3′ UTRs Based on the cell cycle-dependent regulation of HuR localization (Figure 2B) and the changes in cyclin mRNA levels and stability (Figure 3), we hypothesized that mRNAs encoding cyclins A and B1 were possible targets of HuR, mediating its growth-promoting function. To investigate this possibility directly, we tested whether mRNAs encoding cyclins A, B1, D1 and E (Figure 4A) contained binding sites for HuR by incubating radiolabeled transcripts corresponding to their respective 3′ UTRs with purified recombinant HuR. RNase T1 selection assays showed that cyclin A, cyclin B1 and cyclin D1 harbored HuR-binding sites, as demonstrated by the selection of specific RNA oligonucleotides (Figure 4B). A nitrocellulose-binding assay using recombinant HuR further revealed that HuR has high affinity for transcripts encoding cyclins A, B1 and D1, but is unlikely to bind to cyclin E (Figure 4C). Figure 4.HuR binds transcripts encoding cyclins A, B1 and D1. (A) Schematic representation of mRNAs encoding cyclins D1, E, A and B1. Small circles represent AUUUA elements. (B) RNase T1 selection assay. Radiolabeled RNAs were incubated in the presence (+) or absence (–) of 10 nM GST–HuR, then digested with RNase T1. (C) Nitrocellulose filter binding assays were performed after incubating radiolabeled transcripts with the indicated concentrations of GST–HuR; percentages of bound RNA are shown. Download figure Download PowerPoint Binding to these transcripts was then investigated using lysates prepared from synchronous RKO cultures at the times indicated after serum addition. Lysates were incubated with radiolabeled transcripts and the formation of protein–RNA complexes examined. As shown (Figure 5), incubation of cytoplasmic fractions with the coding region of each of the mRNAs studied revealed no protein–RNA complexes. In contrast, 3′ UTR transcripts corresponding to cyclins A, B1 and D1 exhibited abundant complex formation. While complexes forming with the cyclin D1 3′ UTR transcript remained essentially unchanged throughout the time course studied, binding to 3′ UTR transcripts of cyclins A and B1 was largely dependent on the cell cycle stage. The most abundant binding was seen between 12 and 24 h, a time period coinciding with the cells' progression through the S and G2/M phases, when the half-life of mRNAs encoding cyclins A and B1 is the longest (Figure 3B). Little complex formation was observed with the cyclin E 3′ UTR. When nuclear lysates were assayed, all transcripts formed complexes, but no time dependence of binding was seen (not shown). In conclusion, the 3′ UTR of mRNAs encoding cyclins A and B1 forms complexes through binding to RKO cytoplasmic proteins in a cell cycle-dependent manner. Figure 5.Cell cycle-dependent binding to transcripts encoding cyclins A and cyclin B1. Binding activity of radiolabeled RNAs corresponding to either the coding regions or 3′ UTRs of cyclins D1, E, A and B1, and proteins present in cytoplasmic lysates of asynchronous (asyn.) or synchronous RKO cultures after addition of serum for the times indicated is shown. Download figure Download PowerPoint To test directly whether endogenous HuR bound the 3′ UTRs of cyclins A and B1, we carried out supershift analyses in which the migration of HuR–RNA complexes (but not that of other protein–mRNA complexes) is altered by the binding of a monoclonal antibody (Wang et al., 2000). Using this approach, we observed that both HuR–cyclin A 3′ UTR and HuR–cyclin B1 3′ UTR complexes formed with lysates from serum-stimulated RKO cells (Figure 6A); no supershifted complexes were observed with non-specific antibodies. Likewise, no supershifted complexes were seen when using 3′ UTR transcripts from cyclins D1 or E (not shown), suggesting that, under these experimental conditions, HuR does not bind cyclin D1 (a somewhat unexpected observation given its many AUUUA motifs and its binding to recombinant HuR in vitro) or cyclin E mRNAs. Of the bands that routinely formed with the UTRs of cyclins A and B1, some were more clearly depleted by the HuR antibody, suggesting that HuR forms part of those particular complexes. The bands that are not supershifted by the HuR antibody may contain other RNA-binding proteins also recognizing their 3′ UTRs. Alternatively, they may represent post-translational modifications of HuR, or associations of HuR with other proteins that mask the HuR epitope recognized by this monoclonal antibody (although it was generated against the N-terminus to avoid this problem). Finally, after incubation with RKO cell lysates, biotinylated RNAs that encompassed the 3′ UTRs of cyclins A and B1 effectively ‘pulled down’ HuR (described in Materials and methods), as detected by Western blot analysis. Irrelevant biotinylated transcripts, such as RNAs complementary to cyclin A and B1 3′ UTRs (Figure 6B), or RNAs encompassing each coding region (not shown), did not pull down HuR. Figure 6.HuR forms complexes with cyclin A and cyclin B1 3′ UTR transcripts. (A) Complexes forming with lysates from synchronous RKO zeo cells (18 h serum stimulated) and radiolabeled 3′ UTR transcripts from cyclins A and B1 were assayed for their ability to be supershifted by the indicated antibodies. (B) Biotinylated transcripts (sense, 3′ UTR; antisense, complementary to each 3′ UTR) corresponding to the 3′ UTRs of either cyclin A or cyclin B1 were incubated with cytoplasmic RKO lysates (prepared 0 or 18 h after serum stimulation). Complexes were pulled down using streptavidin-conjugated magnetic beads and analyzed by Western blotting to assess HuR levels. lys., 35 μg of RKO zeo whole-cell lysate. Download figure Download PowerPoint RKO ASHuR cells exhibit reduced binding to transcripts from cyclins A and B1 and decreased cyclin A and B1 mRNA stability, mRNA levels and protein expression To examine further the significance of HuR's binding to the 3′ UTR of cyclins A and B1, complexes forming in ASHuR-expressing cells were studied. As shown, the cell cycle-dependent increase in binding to the 3′ UTRs of either cyclin A or cyclin B1 was greatly diminished when assaying lysates from AS.17 and AS.11 (quantitated in Figure 7A). Supershifting of complexes was markedly reduced in ASHuR cells (Figure 7B, +HuR). In summary, cells with reduced HuR expression exhibit substantially reduced binding to transcripts of cyclins A and B1 as the cell progresses through its proliferative cycle. Figure 7.Binding of RKO ASHuR cells to transcripts from cyclins A and B1. (A) Quantitation of radioactive signals (sum of all bands, represented as the mean ± SEM from three independent experiments) from binding experiments similar to those shown in (B). (B) Representative complexes forming between cytoplasmic lysates from synchronous populations of RKO zeo or ASHuR cells (prepared at the times indicated after serum stimulation), and radiolabeled cyclin A 3′ UTR (top) or cyclin B1 3′ UTR (bottom). Supershift analysis was carried out where indicated (+HuR); the arrowhead shows supershifted complexes. Download figure Download PowerPoint A number of studies support the notion that HuR plays a protective role in mRNA stability and enhances mRNA half-life (Jain et al., 1997; Fan and Steitz, 1998a; Wang et al., 2000). To investigate whether HuR also had a protective influence on mRNA turnover in the cell cycle progression paradigm discussed here, we measured the half-lives of mRNAs encoding cyclins A and B1 in RKO ASHuR cells. As observed, the half-life values measured in AS.11 cells (Figure 8A) were significantly lower than those measured in RKO zeo cells (Figure 3B). For example, mRNA half-lives for cyclins A and B1 in asynchronous cultures were 5.5 and 5 h, respectively, in RKO zeo, while they were 3.5 and 3 h, respectively, in AS.11 cells. Likewise, at almost all time points after serum stimulation examined, cyclins A and B1 in RKO zeo cells had longer lived mRNAs than did AS.11 cells. Thus, the half-life of cyclin A mRNA in RKO zeo and AS.11 cells was, respectively, 12 versus 2.5 h (6 h time point), 14 versus 5 h (12 h time point) and 18 versus 12 h (18 h time point). The half-life of cyclin B1 mRNA was also longer in RKO zeo than AS.11 cells at all time points examined: 8 versus 3 h (6 h time point), 12.7 versus 5.2 h (12 h time point), 18 versus 10 h (18 h time point), 13.2 versus 5.2 h (24 h time point) and 11 versus 5 h (32 h time point). The cyclin A mRNA half-life was somewhat shorter in RKO zeo cells at late time points after serum release, possibly due to additional compensatory mRNA-stabilizing factors upregulated in AS.11 during the late stages of the cell's proliferative cycle. Figure 8.Stability and levels of mRNAs encoding cyclins A and B1. (A) Half-lives of mRNAs encoding cyclins A and B1 in AS.11 populations were calculated as described in Figure 3B. mRNA half-lives at each time point following serum stimulation are indicated in parentheses. (B) Relative steady-state levels of mRNAs encoding cyclins A and B1 in RKO untransfected (untr.), zeo, AS.17 and AS.11 cells. Data represent the mean ± SEM from eight independent Northern blots. Download figure Download PowerPoint These changes in mRNA stability, in turn, influenced the steady-state levels of mRNAs encoding cyclins A and B1 (Figure 8B), as they were reduced overall in asynchronous populations of AS.l7 and AS.11 cells (and other ASHuR lines examined; not shown). Finally, cell cycle-dependent expression of cyclins A and B1 was also diminished (Figure 9A), and consequently the kinase activity associated with either cdk2 or cdc2 decreased in cells with reduced HuR expression (Figure 9B). Figure 9.Cyclin A and B1 protein levels and cdk2- and cdc2-associated kinase activity in ASHuR cells. (A) Representative Western blots of cyclin A and cyclin B1 in whole-cell lysates prepared from synchronous RKO zeo and AS.11 cells at the times indicated after serum stimulation. Actin signals served to monitor the equality of sample loading and transfer. (B) Representative cdk2 and cdc2 kinase activities in RKO zeo and ASHuR cells after serum stimulation for the times indicated. Phosphorylated histone H1 is shown. Download figure Download PowerPoint In summary, cells with markedly reduced HuR display lower steady-state levels of cyclin A mRNA and cyclin B1 mRNA, decreased levels of each protein, and reduced activity of the cyclin-dependent kinases regulated by cyclins A and B1. T" @default.
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• W1993691900 date "2000-05-15" @default.
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• W1993691900 title "HuR regulates cyclin A and cyclin B1 mRNA stability during cell proliferation" @default.
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• W1993691900 doi "https://doi.org/10.1093/emboj/19.10.2340" @default.
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