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• W1993764672 abstract "Mercurated DNA was synthesized in vitro by substituting Hg-dCTP or Hg-dUMP for dCTP in one strand of M13mp8DNA in a DNA polymerase I reaction. Restriction enzymes, including Sma I, Pst I, Bam HI, Hind III, and Hinc II, were completely inactive when their recognition sites were fully substituted with Hg-dCMP, while Hg-dUMP containing DNA was hydrolyzed to some extent. Under conditions favoring star activities, or when DNA was substituted with a low level of mercury-nucleotide, DNA was cleaved by restriction enzymes. Susceptibility to degrading and synthesizing enzymes and insensitivity to restriction endonucleases of fully mercurated DNA makes mercuration an attractive molecular tag for in vitro manipulation and selective isolation of Hg-DNA." @default.
• W1993764672 created "2016-06-24" @default.
• W1993764672 creator A5008396172 @default.
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• W1993764672 date "1995-05-01" @default.
• W1993764672 modified "2023-10-16" @default.
• W1993764672 title "Effect of Mercury Substitution of DNA on Its Susceptibility to Cleavage by Restriction Endonucleases" @default.
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• W1993764672 doi "https://doi.org/10.1089/dna.1995.14.445" @default.
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