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• W1993806599 abstract "The variety of biogenesis pathways for small nucleolar RNAs (snoRNAs) reflects the diversity of their genomic organization. We have searched for yeast snoRNAs which are affected by the depletion of the yeast ortholog of bacterial RNase III, Rnt1. In a yeast strain inactivated for RNT1, almost half of the snoRNAs tested are depleted with significant accumulation of monocistronic or polycistronic precursors. snoRNAs from both major families of snoRNAs (C/D and H/ACA) are affected by RNT1 disruption. In vitro, recombinant Rnt1 specifically cleaves pre-snoRNA precursors in the absence of other factors, generating intermediates which require the action of other enzymes for processing to the mature snoRNA. Most Rnt1 cleavage sites fall within potentially double-stranded regions closed by tetraloops with a novel consensus sequence AGNN. These results demonstrate that biogenesis of a large number of snoRNAs from the two major families of snoRNAs requires a common RNA endonuclease and a putative conserved structural motif." @default.
• W1993806599 created "2016-06-24" @default.
• W1993806599 creator A5049057337 @default.
• W1993806599 creator A5064028987 @default.
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• W1993806599 date "1998-12-01" @default.
• W1993806599 modified "2023-10-16" @default.
• W1993806599 title "Yeast RNase III as a key processing enzyme in small nucleolar RNAs metabolism" @default.
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• W1993806599 doi "https://doi.org/10.1006/jmbi.1998.2237" @default.
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