Matches in SemOpenAlex for { <https://semopenalex.org/work/W1993846124> ?p ?o ?g. }
- W1993846124 endingPage "601" @default.
- W1993846124 startingPage "594" @default.
- W1993846124 abstract "Differentiation of skeletal myoblasts is accompanied by induction of a series of tissue-specific genes whose products are required for the specialized functions of the mature muscle fiber. The program for myogenic differentiation is subject to negative control by several peptide growth factors and by the products of mutationally activated ras oncogenes, which persistently activate intracellular cascades normally triggered by specific growth factors. Previously, we reported that induction of the muscle creatine kinase (mck) gene during myogenesis was dependent on a distal upstream enhancer that cooperated with a proximal promoter to direct high levels of expression in developing muscle cells (E. A. Sternberg, G. Spizz, W. M. Perry, D. Vizard, T. Weil, and E. N. Olson, Mol. Cell. Biol. 8:2896-2909). To investigate the mechanisms whereby ras blocks the induction of muscle-specific genes, we have examined the ability of mck 5' regulatory elements to direct expression of the linked reporter gene for chloramphenicol acetyltransferase (cat) in C2 myoblasts bearing mutant N-ras and H-ras oncogenes. In this paper we report that expression of activated ras alleles abolishes activity of the mck upstream enhancer but does not affect the activity of the mck promoter. The ability of ras to repress the expression of mck-cat fusion genes that have been transfected either transiently or stably into myoblasts suggests that ras may exert its effects on muscle-specific genes through mechanisms independent of chromatin configurations or DNA methylation. These results also suggest that ras blocks establishment of the myogenic phenotype by preventing the accumulation of regulatory factors required for transcriptional induction of muscle-specific genes." @default.
- W1993846124 created "2016-06-24" @default.
- W1993846124 creator A5005425937 @default.
- W1993846124 creator A5019468860 @default.
- W1993846124 creator A5044296977 @default.
- W1993846124 creator A5084182062 @default.
- W1993846124 date "1989-02-01" @default.
- W1993846124 modified "2023-09-27" @default.
- W1993846124 title "A ras-dependent pathway abolishes activity of a muscle-specific enhancer upstream from the muscle creatine kinase gene." @default.
- W1993846124 cites W1496519351 @default.
- W1993846124 cites W1506352984 @default.
- W1993846124 cites W1509427554 @default.
- W1993846124 cites W1516540884 @default.
- W1993846124 cites W1531876940 @default.
- W1993846124 cites W1616429637 @default.
- W1993846124 cites W1751903860 @default.
- W1993846124 cites W1774326122 @default.
- W1993846124 cites W1780939239 @default.
- W1993846124 cites W1817814372 @default.
- W1993846124 cites W1858393860 @default.
- W1993846124 cites W1886964719 @default.
- W1993846124 cites W1933402656 @default.
- W1993846124 cites W1950967650 @default.
- W1993846124 cites W1965372133 @default.
- W1993846124 cites W1970823660 @default.
- W1993846124 cites W1972984824 @default.
- W1993846124 cites W1976145475 @default.
- W1993846124 cites W1978274173 @default.
- W1993846124 cites W1981941073 @default.
- W1993846124 cites W1987726188 @default.
- W1993846124 cites W1988096490 @default.
- W1993846124 cites W1999275756 @default.
- W1993846124 cites W2007971883 @default.
- W1993846124 cites W2010488494 @default.
- W1993846124 cites W2014669590 @default.
- W1993846124 cites W2023023900 @default.
- W1993846124 cites W2023150384 @default.
- W1993846124 cites W2026892501 @default.
- W1993846124 cites W2028519621 @default.
- W1993846124 cites W2038695091 @default.
- W1993846124 cites W2042174183 @default.
- W1993846124 cites W2042454416 @default.
- W1993846124 cites W2045739241 @default.
- W1993846124 cites W2046473834 @default.
- W1993846124 cites W2051156045 @default.
- W1993846124 cites W2057094603 @default.
- W1993846124 cites W2058333572 @default.
- W1993846124 cites W2058744360 @default.
- W1993846124 cites W2059944380 @default.
- W1993846124 cites W2065951262 @default.
- W1993846124 cites W2066772600 @default.
- W1993846124 cites W2067613864 @default.
- W1993846124 cites W2069584591 @default.
- W1993846124 cites W2072870234 @default.
- W1993846124 cites W2076277128 @default.
- W1993846124 cites W2083405476 @default.
- W1993846124 cites W2088876976 @default.
- W1993846124 cites W2094267240 @default.
- W1993846124 cites W2094362923 @default.
- W1993846124 cites W2101467051 @default.
- W1993846124 cites W2117616937 @default.
- W1993846124 cites W2118193978 @default.
- W1993846124 cites W2118929543 @default.
- W1993846124 cites W2128746574 @default.
- W1993846124 cites W2128940002 @default.
- W1993846124 cites W2135921792 @default.
- W1993846124 cites W2144206181 @default.
- W1993846124 cites W2144634347 @default.
- W1993846124 cites W2144768031 @default.
- W1993846124 cites W2148429531 @default.
- W1993846124 cites W2151839863 @default.
- W1993846124 cites W2165220352 @default.
- W1993846124 cites W2178383308 @default.
- W1993846124 cites W2247736846 @default.
- W1993846124 cites W2259360768 @default.
- W1993846124 cites W2310708408 @default.
- W1993846124 cites W77825559 @default.
- W1993846124 cites W2739782217 @default.
- W1993846124 doi "https://doi.org/10.1128/mcb.9.2.594" @default.
- W1993846124 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/362636" @default.
- W1993846124 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2651901" @default.
- W1993846124 hasPublicationYear "1989" @default.
- W1993846124 type Work @default.
- W1993846124 sameAs 1993846124 @default.
- W1993846124 citedByCount "31" @default.
- W1993846124 crossrefType "journal-article" @default.
- W1993846124 hasAuthorship W1993846124A5005425937 @default.
- W1993846124 hasAuthorship W1993846124A5019468860 @default.
- W1993846124 hasAuthorship W1993846124A5044296977 @default.
- W1993846124 hasAuthorship W1993846124A5084182062 @default.
- W1993846124 hasBestOaLocation W19938461242 @default.
- W1993846124 hasConcept C104317684 @default.
- W1993846124 hasConcept C111936080 @default.
- W1993846124 hasConcept C150194340 @default.
- W1993846124 hasConcept C153911025 @default.
- W1993846124 hasConcept C161733203 @default.
- W1993846124 hasConcept C165864922 @default.
- W1993846124 hasConcept C207200792 @default.