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- W1993897284 abstract "Abstract Recombination-deficient (Rec − ) mutants of Salmonella typhimurium were used to study special transduction by phage P22 in the absence of the usual background of general transduction. Rec − strains could not be transduced for any marker with a lysate arising by infection. When the same strains were infected with a lysate obtained by induction, low but significant levels of transduction were observed for the pro A and pro B loci (located to one side of the prophage attachment site), but not for pro C (located to the other side of the attachment site), leu , or thy . Integration-deficient ( int ) phage lysates were unable to transduce the pro A and pro B loci in Rec − cells unless int + helper phage were present. These results suggest that although Rec − cells cannot undergo general transduction, special transduction (by lysogenization) for the pro A and pro B loci is found. A mixture of int − and int + phage were unable to transduce the pro C locus despite the large number of pro C -transducing particles present in an int lysate. The inability of the int + phage genomes to promote successful lysogenization of pro C -transducing particles in Rec − cells may indicate a requirement for a structural element near the left prophage end for integration of phage P22. Alternatively, the deletion of c 2 , the structural gene for P22 repressor, from most pro C -transducing particles may prevent successful lysogenization by these particles. Finally, the pro C locus may lie too far away from the prophage site to be included in special transducing particles containing any phage DNA." @default.
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- W1993897284 date "1968-10-01" @default.
- W1993897284 modified "2023-09-26" @default.
- W1993897284 title "Transduction by phage P22 in a recombination-deficient mutant of Salmonella typhimurium" @default.
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- W1993897284 doi "https://doi.org/10.1016/0042-6822(68)90144-x" @default.
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