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- W1993919493 abstract "A cDNA library was constructed in pBR322 from 18S to 20S mRNA that was extracted from human kidney cells, frasctionated on oligo(dT)-cellulose column and sucrose-density gradient, and confirmed for urokinase production in Xenopus laevis oocytes. The Escherichia coli RR1 transformants were hybridized to synthetic oligonucleotide probe prepared according to the known amino acid sequence, Glu 73 to Glu 77 of human urinary urokinase chain B. The entire cloned cDNA covers a 2250-bp region, wherein the 1293-bp sequence codes for preprourokinase consisting of 431 amino acids, with the first 20 residues being a signal peptide. The 5'-untranslated region is at least 80 bp long and the 3'-untranslated region is longer than 850 bp." @default.
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- W1993919493 date "1985-01-01" @default.
- W1993919493 modified "2023-09-28" @default.
- W1993919493 title "Molecular cloning of cDNA coding for human preprourokinase" @default.
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- W1993919493 doi "https://doi.org/10.1016/0378-1119(85)90084-8" @default.
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