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- W1993972938 abstract "Bacillus subtills plasmid pGR71 Is a promoter-probe shuttle vector derived from pUB110(1). The expression of the cat gene on pGR71 in B. subtilis requires the insertion of a Bacillus promoter and a ribosomal binding site (RBS) into the HindIII cloning site Immediately upstream from the cat gene. A recombinant plasmid of pGR71, named pGR7l-369, was obtained by a spontaneous deletion of a fragment containing most of the inserted Hindill fragment and the replication origin necessary for multiplication in Escherlchia coil. The expression of the cat gene in B. subtilis cells carrying this plasmid was inducible by heat. Nucleotide sequence analysis of the upstream region of the cat gene, deletion analysis, and dot blot hybridization analysis of mRNA in various conditions revealed that the cat gene was expressed by heat-inducible translational coupling and that the regulatory region of heat inducibility was present in the upstream region of the cat gene." @default.
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- W1993972938 date "1990-01-01" @default.
- W1993972938 modified "2023-09-26" @default.
- W1993972938 title "Heat-inducible translational coupling inBacillus subtilis" @default.
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- W1993972938 doi "https://doi.org/10.1093/nar/18.4.739" @default.
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