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- W1993983219 abstract "ABSTRACT The liberation of arachidonic acid (AA), by phospholipase A 2 (PLA 2 ), is the rate-limiting step in a number of cell signalling pathways. In the pancreatic β-cell, AA itself is thought to participate in the regulation of insulin secretion. Recently a Ca 2+ -sensitive, AA-selective cytosolic PLA 2 (type IV cPLA 2 ) has been isolated from the human monocyte U937 cell line. Although the DNA sequence of this enzyme implies a molecular weight of 85 kDa, the protein migrates with a molecular weight of 100-110 kDa on SDS-PAGE. In many cell types, cPLA 2 s which are reactive towards antibodies raised against the type IV cPLA 2 have been shown to hydrolyse AA from membrane glycerophospholipids. Using a polyclonal antibody raised against a recombinant form of type IV cPLA 2 , we have detected an immunoreactive protein with a molecular weight of 93·5 kDa in rat islets of Langerhans. Furthermore, we have detected similar immunoreactive proteins in insulin-secreting β-cell lines and have shown co-expression of type IV cPLA 2 immunoreactivity and insulin immunoreactivity in rat pancreatic β-cells. Under non-stimulatory conditions the 93·5 kDa immunoreactive protein detected in rat islets of Langerhans was located predominantly in the cytosolic fraction. We have shown that immunoprecipitation of the rat immunoreactive protein from rat islet homogenates significantly decreases the total dithiothreitol/β-mercaptoethanol-insensitive PLA 2 activity by 56·4±7% This provides further evidence that the immunoreactive rat protein is a type IV cPLA 2 and is responsible for a large component of the PLA 2 activity in rat islets of Langerhans. It is possible that, in the rat β-cell, type IV cPLA 2 couples the increase in intracellular Ca 2+ , brought about by insulin secretagogues, to the liberation of AA and the subsequent release of insulin." @default.
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- W1993983219 date "1996-08-01" @default.
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- W1993983219 title "Identification and localisation of a type IV cytosolic phospholipase A2 in rat pancreatic β-cells" @default.
- W1993983219 doi "https://doi.org/10.1677/jme.0.0170031" @default.
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