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- W1994071822 abstract "Investigation of single molecule DNA dynamics in confined environments has led to important applications in DNA analysis, separation, and sequencing. Here, we studied the electrophoretic transport of DNA molecules through nanochannels shorter than the DNA contour length and calculated the associated translocation time curves. We found that the longer T4 DNA molecules required a longer time to traverse a fixed length nanochannel than shorter λ DNA molecules and that the translocation time decreased with increasing electric field which agreed with theoretical predictions. We applied this knowledge to design an asymmetric electric pulse and demonstrate the different responses of λ and T4 DNA to the pulses. We used Brownian dynamics simulations to corroborate our experimental results on DNA translocation behaviour. This work contributes to the fundamental understanding of polymer transport through nanochannels and may help in designing better separation techniques in the future." @default.
- W1994071822 created "2016-06-24" @default.
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- W1994071822 date "2014-03-01" @default.
- W1994071822 modified "2023-09-23" @default.
- W1994071822 title "DNA translocation through short nanofluidic channels under asymmetric pulsed electric field" @default.
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- W1994071822 doi "https://doi.org/10.1063/1.4871595" @default.
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