Matches in SemOpenAlex for { <https://semopenalex.org/work/W1994099538> ?p ?o ?g. }
- W1994099538 endingPage "25688" @default.
- W1994099538 startingPage "25683" @default.
- W1994099538 abstract "Glia maturation factor (GMF) is a member of the actin-depolymerizing factor (ADF)/cofilin family. ADF/cofilin promotes disassembly of aged actin filaments, whereas GMF interacts specifically with Arp2/3 complex at branch junctions and promotes debranching. A distinguishing feature of ADF/cofilin is that it binds tighter to ADP-bound than to ATP-bound monomeric or filamentous actin. The interaction is also regulated by phosphorylation at Ser-3 of mammalian cofilin, which inhibits binding to actin. However, it is unknown whether these two factors play a role in the interaction of GMF with Arp2/3 complex. Here we show using isothermal titration calorimetry that mammalian GMF has very low affinity for ATP-bound Arp2/3 complex but binds ADP-bound Arp2/3 complex with 0.7 μM affinity. The phosphomimetic mutation S2E in GMF inhibits this interaction. GMF does not bind monomeric ATP- or ADP-actin, confirming its specificity for Arp2/3 complex. We further show that mammalian Arp2/3 complex nucleation activated by the WCA region of the nucleation-promoting factor N-WASP is not affected by GMF, whereas nucleation activated by the WCA region of WAVE2 is slightly inhibited at high GMF concentrations. Together, the results suggest that GMF functions by a mechanism similar to that of other ADF/cofilin family members, displaying a preference for ADP-Arp2/3 complex and undergoing inhibition by phosphorylation of a serine residue near the N terminus. Arp2/3 complex nucleation occurs in the ATP state, and nucleotide hydrolysis promotes debranching, suggesting that the higher affinity of GMF for ADP-Arp2/3 complex plays a physiological role by promoting debranching of aged branch junctions without interfering with Arp2/3 complex nucleation." @default.
- W1994099538 created "2016-06-24" @default.
- W1994099538 creator A5056771831 @default.
- W1994099538 creator A5063558144 @default.
- W1994099538 creator A5079124124 @default.
- W1994099538 date "2013-09-01" @default.
- W1994099538 modified "2023-10-16" @default.
- W1994099538 title "Glia Maturation Factor (GMF) Interacts with Arp2/3 Complex in a Nucleotide State-dependent Manner" @default.
- W1994099538 cites W1606348378 @default.
- W1994099538 cites W1635520329 @default.
- W1994099538 cites W1963982237 @default.
- W1994099538 cites W1969911260 @default.
- W1994099538 cites W1972167969 @default.
- W1994099538 cites W1974086731 @default.
- W1994099538 cites W1976745001 @default.
- W1994099538 cites W1983813017 @default.
- W1994099538 cites W1984559046 @default.
- W1994099538 cites W1985210076 @default.
- W1994099538 cites W1990311552 @default.
- W1994099538 cites W1991878174 @default.
- W1994099538 cites W1994201298 @default.
- W1994099538 cites W1995517865 @default.
- W1994099538 cites W1996002116 @default.
- W1994099538 cites W1999619602 @default.
- W1994099538 cites W2002255871 @default.
- W1994099538 cites W2007586038 @default.
- W1994099538 cites W2021087020 @default.
- W1994099538 cites W2023808883 @default.
- W1994099538 cites W2027126201 @default.
- W1994099538 cites W2045457002 @default.
- W1994099538 cites W2045923054 @default.
- W1994099538 cites W2046954672 @default.
- W1994099538 cites W2058497997 @default.
- W1994099538 cites W2063065938 @default.
- W1994099538 cites W2069441617 @default.
- W1994099538 cites W2070791254 @default.
- W1994099538 cites W2074853505 @default.
- W1994099538 cites W2079244641 @default.
- W1994099538 cites W2082677295 @default.
- W1994099538 cites W2084746099 @default.
- W1994099538 cites W2093196157 @default.
- W1994099538 cites W2104214146 @default.
- W1994099538 cites W2105756079 @default.
- W1994099538 cites W2108417233 @default.
- W1994099538 cites W2108582887 @default.
- W1994099538 cites W2111894578 @default.
- W1994099538 cites W2112928445 @default.
- W1994099538 cites W2113395636 @default.
- W1994099538 cites W2120446093 @default.
- W1994099538 cites W2122782850 @default.
- W1994099538 cites W2128212489 @default.
- W1994099538 cites W2128535856 @default.
- W1994099538 cites W2137861623 @default.
- W1994099538 cites W2146669392 @default.
- W1994099538 cites W2154637838 @default.
- W1994099538 cites W2171122741 @default.
- W1994099538 cites W2171956751 @default.
- W1994099538 cites W4232213593 @default.
- W1994099538 doi "https://doi.org/10.1074/jbc.c113.493338" @default.
- W1994099538 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3764776" @default.
- W1994099538 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23897816" @default.
- W1994099538 hasPublicationYear "2013" @default.
- W1994099538 type Work @default.
- W1994099538 sameAs 1994099538 @default.
- W1994099538 citedByCount "28" @default.
- W1994099538 countsByYear W19940995382014 @default.
- W1994099538 countsByYear W19940995382015 @default.
- W1994099538 countsByYear W19940995382016 @default.
- W1994099538 countsByYear W19940995382018 @default.
- W1994099538 countsByYear W19940995382019 @default.
- W1994099538 countsByYear W19940995382020 @default.
- W1994099538 countsByYear W19940995382021 @default.
- W1994099538 countsByYear W19940995382022 @default.
- W1994099538 countsByYear W19940995382023 @default.
- W1994099538 crossrefType "journal-article" @default.
- W1994099538 hasAuthorship W1994099538A5056771831 @default.
- W1994099538 hasAuthorship W1994099538A5063558144 @default.
- W1994099538 hasAuthorship W1994099538A5079124124 @default.
- W1994099538 hasBestOaLocation W19940995381 @default.
- W1994099538 hasConcept C11960822 @default.
- W1994099538 hasConcept C125705527 @default.
- W1994099538 hasConcept C142669718 @default.
- W1994099538 hasConcept C1491633281 @default.
- W1994099538 hasConcept C2776414213 @default.
- W1994099538 hasConcept C2776601116 @default.
- W1994099538 hasConcept C2993400109 @default.
- W1994099538 hasConcept C55493867 @default.
- W1994099538 hasConcept C86803240 @default.
- W1994099538 hasConcept C95444343 @default.
- W1994099538 hasConceptScore W1994099538C11960822 @default.
- W1994099538 hasConceptScore W1994099538C125705527 @default.
- W1994099538 hasConceptScore W1994099538C142669718 @default.
- W1994099538 hasConceptScore W1994099538C1491633281 @default.
- W1994099538 hasConceptScore W1994099538C2776414213 @default.
- W1994099538 hasConceptScore W1994099538C2776601116 @default.
- W1994099538 hasConceptScore W1994099538C2993400109 @default.
- W1994099538 hasConceptScore W1994099538C55493867 @default.
- W1994099538 hasConceptScore W1994099538C86803240 @default.